【作者】 陈冠豪；

【导师】 孙广仁；

【作者基本信息】 山东中医药大学 ， 中医基础理论， 2010， 博士

【摘要】 目的：从肺主气的理论上,对肺癌的发生机制进行系统的阐释与论证,以丰富肺癌的防治。通过建立小鼠Lewis肺癌模型,观察黄芪注射液对小鼠生存时间,小鼠瘤组织的重量及抑瘤率的影响。采用免疫组化方法,观察黄芪注射液对小鼠肿瘤坏死因子(TNF-α)及基质金属蛋白酶-9(MMP-9)的影响。从微观机制探讨黄芪注射液对小鼠肺癌的抑制和转移的作用机制,以期在临床上能有效的运用黄芪注射液对肺癌治疗提供实验依据。方法：建立小鼠Lewis肺癌转移模型,采用中西药对比进行治疗,观察黄芪注射液对小鼠瘤组织的影响。通过观察小鼠的一般情况、体重改变、抑瘤率、肺组织内基质金属蛋白酶MMP-9和肿瘤坏死因子TNF-α等细胞因子的变化。从细胞分子水平阐释这些变化机制,探讨黄芪注射液对肺癌的作用机制的相关性,为防治肺癌提供新思路新治法及理论依据。结果：小鼠Lewis肺癌转移模型符合肺癌的临床表现,一般情况与肺癌的临床症状相符,各项指标的变化也与肺癌的指标变化相一致。黄芪注射液对小鼠移植性肺癌及小鼠肺转移均有明显的抑制作用(P<0.05),并且黄芪注射液对小鼠免疫功能有显著的提高。黄芪注射液也能降低TNF-α及MMP-9的表达量(P<0.05)。结论：黄芪注射液对小鼠肺癌具有明显抑制作用；能提高肺癌小鼠机体免疫功能作用,表明黄芪注射液在小鼠肺癌的发生和转移过程中起着重要的干预作用。黄芪注射液能明显降低肿瘤坏死因子(TNF-α)及基质金属蛋白酶-9的表达量,表明黄芪注射液在一定程度上能够防治肺癌。更多还原

【Abstract】 Objective:Froming the lungs the main gas theory, this thesis expounds the mechanism of lung cancer a systematic explanation and demonstration in order to enrich the prevention and treatment of lung cancer. Through the establishment of mouse Lewis lung cancer model. Observation of Astragalus injection on survival time of mice, mice tumor weight and inhibition rate of metastasis of lung cancer in mice. Immunohistochemical method used to observe the astragalus on the mouse tumor necrosis factor (TNF-a) and matrix metalloproteinase -9 (MMP-9) impact. From the micro-mechanism of Astragalus injection on the suppression of lung cancer in mice and transfer mechanism of action with a view to effective clinical use of astragalus to provide experimental basis for the treatment of lung cancer.Methods:To establish a mouse Lewis lung metastasis model, using Chinese and Western medicines for treatment compared to observe the astragalus on the impact of tumor tissue in mice. By observing the general situation in mice, weight change, inhibition rate, the lung tissue matrix metalloproteinase MMP-9 and tumor necrosis factor TNF-a and other cytokines. From the cellular and molecular level to explain the mechanism of these changes and explore the role of Astragalus injection on the relevance of lung cancer for the prevention and treatment of lung cancer to provide new ideas and theoretical basis.Results:The mouse Lewis lung metastasis model of lung cancer in line with the clinical performance of general clinical symptoms of lung cancer and consistent with changes in the indicators also consistent with changes in indicators of lung cancer. Astragalus injection on mice transplanted lung cancer and pulmonary metastasis in mice were significantly inhibited (P<0.05), and astragalus injection on immune function significantly improved. Astragalus also reduce TNF-αand MMP-9 protein expression (P <0.05).Conclusion:Astragalus injection has obvious inhibitory effect on mouse lung cancer; can enhance immune function of lung cancer in mice, showing that the occurrence of Astragalus injection in mice, and metastasis of lung cancer plays an important role in the intervention. Astragalus injection can significantly reduce the tumor necrosis factor (TNF-α) and matrix metalloproteinase -9 expression levels, indicating a certain degree of Astragalus injection in prevention and treatment of lung cancer can be.更多还原