【作者】 刘波；

【导师】 唐强；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2010， 博士

【摘要】 目的观察头穴丛刺结合环境刺激对缺氧缺血性脑损伤新生大鼠海马微管相关蛋白-2和突触素表达及突触超微结构等的影响,探讨头穴丛刺结合环境刺激对新生大鼠缺氧缺血性脑损伤后神经可塑性影响的物质基础及其发生机制。方法制作HIBD新生动物模型,随机分为五组。采用Morris水迷宫检测学习记忆能力、免疫组化检测各组海马CA1区MAP-2和突触素的表达、透射电镜观察神经元突触超微结构和突触数目。结果(1)头穴丛刺结合环境刺激法能够促进缺氧缺血性脑损伤大鼠学习记忆能力的提高;(2)头穴丛刺结合环境刺激法使海马CA1区MAP-2的表达增高;(3)头穴丛刺结合环境刺激法能够增加海马CA1区突触素的表达;(4)头穴丛刺结合环境刺激法可以减轻HI所致的突触超微结构损伤,改善突触结构神经信息的传递,促进缺血缺氧的脑组织超微结构恢复;(5)头穴丛刺结合环境刺激法可促进突触数目的增加。结论头穴丛刺结合环境刺激法能够促进缺氧缺血性脑损伤大鼠学习记忆能力的提高;海马CA1区MAP-2和突触素表达的增高,缺血缺氧的脑组织超微结构恢复和突触数目的增加,可能参与了头穴丛刺与环境刺激对HIBD神经可塑性的影响机制。更多还原

【Abstract】 Objective To observe the first hole cluster-needling combined environmental stimuli on hypoxic-ischemic brain injury in neonatal rats with hippocampal microtubule-associated protein -2 and synaptophysin expression and synaptic ultrastructure of the impact of the first points of the integration of environmental stimulation on the cluster-needling hypoxic-ischemic brain injury affect the material basis of neural plasticity and its mechanism. Methods Of production of HIBD newborn animals were randomly divided into five groups. Using Morris water maze test of learning and memory capacity of each group was detected by immunohistochemistry in hippocampal CA1 area MAP-2 and synaptophysin expression, transmission electron microscopy observation of neuronal synapses and synaptic ultrastructure number. Results (1) the first hole cluster-needling the integration of environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; (2) the first hole cluster-needling the integration of environmental stimulation of hippocampal CA1 area increased expression of MAP-2; (3) the first hole cluster-needling combined environmental stimuli can increase hippocampal synaptophysin expression in CA1 area; (4) the first hole cluster-needling combined environmental stimuli can reduce HI-induced synaptic ultrastructure injury, to improve the synaptic structure neural transmission of information to promote the hypoxic-ischemic brain tissue ultrastructure recovery; (5) the first hole cluster-needling combined environmental stimuli can promote an increase in the number of synapses. Conclusion Of scalp cluster-needling combined environmental stimulation can contribute to hypoxic-ischemic brain injury in rats with learning and memory capabilities; hippocampal CA1 area MAP-2 and synaptophysin expression increased in hypoxic-ischemic brain ultrastructure to resume and the increase in the number of synapses may be involved in the first cluster-needling acupoint stimulation on HIBD and environmental impact of neural plasticity mechanisms.更多还原