【作者】 颜培宇；

【导师】 唐强；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2010， 博士

【摘要】 目的观察不同穴位针刺对免疫抑制大鼠神经肽相关基因表达影响，探讨其免疫调节机制，阐明针刺对免疫抑制机体神经内分泌免疫网络的作用。方法采用环磷酰胺腹腔注射法制备免疫抑制大鼠模型，通过RT-PCR法观察不同穴位针刺后免疫抑制大鼠脑组织β-EPmRNA、SPmRNA表达的变化；采用We s t e r n - b l o t法观察脾脏淋巴细胞表面β-EPR及SPR表达情况；通过流式细胞仪检测血中CD4+T细胞和CD8+T细胞的含量；采用ELISA法检测针刺对各组大鼠血清IL-4、IFN-γ含量的影响。结果不同穴位针刺能够提高免疫抑制大鼠脑垂体β-EPmRNA、下丘脑SPmRNA表达，治疗各组与模型对照组比较有显著性差异（P <0 . 0 5），头针加足三里、关元组( E组)效果最佳。针刺能够降低外周脾淋巴细胞表面SPR表达，提高外周脾淋巴细胞表面β-EPR表达；同时不同穴位针刺能提高外周血CD4+T细胞含量，治疗各组与模型对照组比较有显著性差异（P <0.05），头针加足三里、关元组效果最佳，与正常组比较无显著性差异（P > 0 . 0 5）、对CD8+T细胞无显著影响，与正常组比较无显著性差异（P> 0 . 0 5）；不同穴位针刺能显著降低免疫抑制大鼠血清IL-4水平，治疗各组与模型对照组比较有显著性差异（P <0.05），头针加足三里、关元组效果最佳，还可提高血清I FN-γ含量,治疗各组与模型对照组比较有显著性差异（P <0 . 0 5），头针加足三里、关元组效果最佳。结论不同穴位针刺可以提高免疫抑制大鼠低下的细胞免疫功能，尤其以头穴加体穴效果最佳。其可能的机理是通过影响神经肽-免疫细胞受体-细胞因子通路完成的。更多还原

【Abstract】 Objective To observe the effect of different acupuncture points in immunosuppressive rats on the neuropeptide gene expression,e-xplore the immu ne regulatory me chanisms,to clarify role of ac-upuncture on the neuroendocrine-immune network.Methods Preparation immunosuppressive rats model by intrape-ritoneal injection of CP .To observedβ-EPmRNA, SPmRNA exp-ression changes in brain tissue of rats by RT-PCR after acupun-cture;observed spleen cell surfaceβ-EPR and SPR expression us-ing western-blot; by FCM observed CD4+T cells and CD8+ T c-ells in blood; using ELISA method to observed IL-4, IFN-γlev-els of serum.Results Different points acupuncture can improveβ-EPmRNA、SPmRNA expression in immunosuppressive rats, compared to mod-el group,there is a significant difference (P<0.05), E group is t-he best. Acupuncture can reduce SPR expression on the splenic lymphocytes surface,improveβ-EPR content; different points acu-puncture can improve peripheral CD4+T cells content , compare-d to model group, there is a significant difference (P<0.05).CD8+T cells had no significant difference. Different points acupuncture can significantly decrease serum IL-4, increase serum IFN-γcontent of immunosuppressive rats, compared to model group,there is a significant difference (P<0.05),E group is the best.Conclusions Acupuncture can improve the cellular-immune funct-oin of i mmunos uppressi ve rats, particular ly the head plus body acupuncture points(E group) is best. The possible mechanis m is that affects neuropeptide-immune cell receptors-cytokins pathway.更多还原