【摘要】 CRISPR/dCas9是以CRISPR/Cas9系统为基础发展而来的转录调控工具.本文综述了CRISPR/dCas9作为转录抑制工具(CRISPRi)和转录激活工具(CRISPRa)的发展及应用,总结了在细菌中应用CRISPR/dCas9系统时转录调控系统的选择、构建、靶位点的选择以及gRNA的设计等方面存在的问题,并对相关解决方法进行了展望.更多还原

【Abstract】 CRISPR/dCas9 is a transcriptional regulation tool developed from the CRISPR/Cas9 system. The development and application of CRISPR/dCas9 as a transcriptional interference(CRISPRi)and transcription activator(CRISPRa)were summarized in this paper. Problems in the application of CRISPR/dCas9 system in bacteria,such as target sites,target selection and construction of the transcriptional regulatory system,the design of gRNA,and possible solutions were also discussed in the paper.更多还原