【摘要】 采用氨氮模拟废水对生物海绵铁体系进行驯化,筛选出一株具有好氧反硝化功能的铁细菌,并对菌株生理生化及反硝化特性进行研究。经BTB培养基、Winogradsky铁细菌固体培养基初筛,结合反硝化性能测定,对菌株进行分离筛选。经形态观察、生理生化鉴定和16S r DNA序列分析,对菌株进行鉴定。研究碳源、海绵铁加量、pH值、温度及C/N对其生长量、反硝化性能的影响。筛选得到一株具有好氧反硝化功能的铁细菌H5,经鉴定为反硝化无色杆菌属(Achromobacter denitrificans sp.)。反硝化特性试验结果表明:该菌最佳碳源、海绵铁投加量、温度、pH值和C/N分别为酒石酸钾钠、1 mg/L、30℃、7、17∶1。该菌在生物脱氮方面具有明显优势。更多还原

【Abstract】 The ammonia sponge nitrogen was used to simulate the biological sponge iron system,and an aerobic denitrification iron bacteria was screened. The physiological and biochemical and denitrification characteristics of the strain were studied. The strains were screened by BTB medium and Winogradsky iron bacteria solid medium,and the denitrification performance was tested. The strain was identified by morphological observation,physiological and biochemical identification and 16 S r DNA sequence analysis. The effects of carbon source,sponge iron dosage,pH value,temperature and C/N on the growth and denitrification performance were studied. A strain of iron bacteria H5 with aerobic denitrification function was screened and identified as Achromobacter denitrificans sp. The denitrification characteristics showed that the optimum carbon source,sponge iron dosage,temperature,pH value and C/N were potassium sodium tartrate,1 mg/L,30 ℃,7,17 ∶ 1,respectively. The results showed that the iron bacteria H5 had obvious advantages in biological nitrogen removal.更多还原