【作者】 丰水强；

【导师】 杨为民；

【作者基本信息】 华中科技大学 ， 外科学， 2013， 硕士

【摘要】 目的：检测女性膀胱过度活动症(OAB)患者膀胱尿路上皮中瞬时电位香草酸受体亚型1(TRPV1)的表达，以期探讨TRPV1在女性OAB患者中的临床意义。方法：采用免疫组织化学SABC法，分别检测21例女性OAB患者膀胱尿路上皮和9例正常女性膀胱尿路上皮中TRPV1的表达，以平均光密度(AOD)表示其相对含量，并结合临床尿流动力学资料进行分析。结果：TRPV1在女性OAB患者膀胱尿路上皮中的表达明显高于正常膀胱尿路上皮,其平均光密度值分别为0.4834±0.1252与0.3658±0.1009，两者比较有显著性差异(P＜0.05)；将女性OAB患者的临床尿流动力学参数值分别与正常女性参考值进行比较，最大尿流率、首次排尿感觉容量、强烈排尿感觉容量、最大膀胱容量以及膀胱顺应性均有显著性差异(p＜0.05)。结论：女性OAB患者膀胱尿路上皮中TRPV1高度表达与OAB的发生发展密切相关，提示膀胱敏感性增高在女性OAB的发病机制中具有重要作用。更多还原

【Abstract】 Objective:To detect the expression of Transient Receptor Potential Vanilloid Type1(TRPVl) receptor in the female overactive bladder (OAB) patient, and to investigate its clinical significance in the female OAB patient.Method:The expressions of TRPV1in21cases of the female OAB patients and9cases of normal bladder were detected by using immunohistochemistry SABC assay, and the intensity of TRPV1expression were quantified by average optical density(AOD), two groups were compared with AOD values and urodynamic datas.Results:The AOD values of TRPV1expression in OAB patients was significantly higher than the normal bladders (respectively,0.4834±0.1252,0.3658±0.1009,P<0.05). We also found the significant difference between the female OAB patients and the healthy women in the urodynamics parameters, which included maximum flow rate, first desire volume, strong desire volume, maximum cystometric capacity and bladder compliance.Conclusions:Compared with the normal bladder, the expression of TRPV1was obviously up-regulation in the female OAB patient. TRPV1may involve in the development of the female OAB, which indicates that bladder hypersens may play an important role in the pathogenesis of female OAB.更多还原