【作者】 张子木；

【导师】 罗凯；

【作者基本信息】 湖北民族大学 ， 林特食品加工与开发， 2022， 硕士

【摘要】 目的:为探索壶瓶碎米荠多糖结构修饰以及修饰后多糖的生物活性变化规律,利用三氧化硫-吡啶法对壶瓶碎米荠多糖进行了硫酸化结构修饰,修饰成功后采用柱层析法进行分离纯化,采用核磁共振等技术研究其主要结构。并通过与玉米醇溶蛋白进行复合制作一种具有抗氧化功能的食品外包装材料。方法:主要研究过程如下:首先通过硫酸化修饰我们得到了五种不同取代度的硫酸化壶瓶碎米荠多糖,取代度分别为0.46、0.55、0.69、0.72、0.80。通过傅立叶变红外光谱初步对其改性效果进行了分析。在此基础上研究了改性壶瓶碎米荠多糖的水溶性和抗氧化活性。从中我们筛选出取代度为0.72的硫酸化壶瓶碎米荠多糖作为主要研究对象将其命名为SCHP。经过进一步纯化后我们得到2种不同多糖,我们将其中主要部分命名为SCHP-Ⅱ。通过高效凝胶色谱、核磁共振等技术分析SCHP-Ⅱ结构同时进行了生物活性研究。最后利用SCHP与玉米醇溶蛋白复合,成功制备出新型生物活性膜并对薄膜进行了功能性和表征研究。结果:通过高效凝胶色谱和核磁等分析出SCHP-Ⅱ主要由葡萄糖与半乳糖组成、SCHP-Ⅱ的Mw为22.2k Da;Mn为13.4k Da。通过二维核磁辅助,分析出SCHP-Ⅱ的主链结构为→4)-ɑ-D-Glc(1→4)-β-D-Galp A-(1→4)-β-D-Galp A-(1→4)-ɑ-Rhap(1→6)-ɑ-D-Glc(1→。同时我们发现通过对体外抗氧化结果表明SCHP比CHP具有更好的抗氧化活性和水溶性。结论:这表明了经过硫酸化修饰的壶瓶碎米荠多糖具有更好的应用前景。在添加不同浓度SCHP后提高了薄膜对羟自由基、ABTS、DPPH的清除能力。将添加4mg/ml SCHP的薄膜比未添加SCHP薄膜相比,在透明度上虽然降低,但在拉伸强度上有一定的提高。这些结果表明Zein-SCHP膜可以作为一种具有抗氧化功能的食品包装材料。更多还原

【Abstract】 Objective :In order to explore the structural modification of Cardamine hupingshanensis polysaccharides(CHP)and the changes of the biological activity of the modified polysaccharide,the sulfated structure of CHP was modified by the sulfur trioxide-pyridine method,and after the successful modification,it was separated and purified by column chromatography,and the main structure was studied by nuclear magnetic resonance and other techniques.The main structure was investigated by NMR.And a food packaging material with antioxidant function was produced by compounding with Zein.The main research process is as follows: firstly,we obtained five different substitution degrees of sulfated Cardamine hupingshanensis polysaccharides(SCHP)by sulfation modification,the substitution degrees were 0.46,0.55,0.69,0.72 and 0.80,respectively.the modification effect was initially analyzed by Fourier transform infrared spectroscopy.On this basis,the water solubility and antioxidant activity of SCHP were investigated.After further purification,we obtained two different polysaccharides,and we named the main part of them as SCHP-II.The structure of SCHP-II was analyzed by GPC and NMR co-contrast techniques and the biological activity was also studied.Finally,we successfully prepared new bioactive films using SCHP compounded with Zein and performed functional and characterization studies on the films.Experimental results: We obtained that SCHP-Ⅱis mainly composed of glucose and galactose,and the Mw of SCHP-Ⅱis 22.2 k Da;Mn is 13.4 k Da.Meanwhile,the main chain structure is→4)-ɑ-D-Glc(1→4)-β-D-Galp A-(1→4)-β-D-Galp A-(1→4)-ɑ-Rhap(1→6)-ɑ-DGlc(1→.We also found that the in vitro antioxidant results showed that SCHP has better antioxidant activity and water solubility than CHP.This indicates that the sulfated modified CHP has better application prospects.The scavenging ability of the films against hydroxyl radicals,ABTS,and DPPH was improved after the addition of different concentrations of SCHP.The films with the addition of 4 mg/ml SCHP showed some improvement in tensile strength,although lower in transparency,compared to the films without the addition of SCHP.These results suggest that Zein-SCHP film can be used as a food packaging material with antioxidant function.更多还原