【摘要】 研究莲子心（plumula nelumbinis）中黄酮苷类大极性成分及其抗氧化活性。选择80%乙醇进行初步提取分离，得到莲子心粗提物。先后选用D001阳离子交换树脂和AB-8大孔树脂对粗提物进行柱层析得到莲子心总黄酮部分。进一步选择硅胶柱、Sephadex LH-20柱、ODS反向柱、制备型HPLC等对莲子心总黄酮部分进行分离纯化，并利用波谱解析和理化性质对黄酮苷类化合物的结构进行鉴定，最终确定了11个化合物为：异牡荆素（isovitexin, 1）、牡荆素（vitexin, 2）、异夏佛托苷（isoshaftoside, 3）、夏佛托苷（shaftoside, 4）、金丝桃苷（hypericin, 5）、芦丁（rutin, 6）、芹菜素-6（apigenin-6）,8-二吡喃葡萄糖苷（8-glucopyranoside, 7）、4-羟基苯乙醇-O-β-D-吡喃葡萄糖苷（icariside D2,8）、忍冬苦苷（lonicerin, 9）、荭草苷（orientin, 10）、异荭草苷（isoorientin, 11）;并对7种黄酮苷类化合物进行了DPPH自由基清除活性测试。化合物8为首次从莲属植物中分离得到；活性测试结果表明，化合物5、6的抗氧化活性较好，当浓度为120μmol·L^-1时，其DPPH自由基清除率达到了和Vc相当的效果。更多还原

【Abstract】 It is to study the flavonoids glycosides in Lianzixin（Plumula nelumbinis） and its antioxidant activity. Choosing 80% ethanol as a solvent for extraction to obtain crude extraction, macroporous resins（D001 and AB-8） were used to perform column chromatography on the crude extraction to obtain the total flavonoids of Lianzixin（Plumula nelumbinis）. Silica gel column, Sephadex LH-20 column, ODS column, prep-HPLC and other methods were selected to purify the total flavonoids. The structures of 11 compounds were determined as follows: Isovitexin（1）,Vitexin（2）,Isoshaftoside（3）,Shaftoside（4）,Hypericin（5）,Rutin（6）,Apigenin-6,8-glucopyranoside（7）,Icariside D2（8）,Lonicerin（9）,orientin（10）,isoorientin（11）. The DPPH free radical scavenging assay of flavonoids were tested on 7 flavonoid glycoside compounds. Compound 8 was obtained from Lianzixin（Plumula nelumbinis） for the first time. The results showed that Compound 5 and 6 had good DPPH radical scavenging ability, and when the concentration was 120 μmol·L^-1,its DPPH radical scavenging rate was equivalent to that of Vc.更多还原