【摘要】 目的观察年龄对大鼠大脑胆碱能神经系统中乙酰胆碱转移酶(choline acetyltransferase,ChAT)与乙酰胆碱酯酶(acetylcholinesterase,AChE)活性的影响。方法Sprague-Dawley雄性大鼠,按照年龄分为4～5周龄组、4～5月龄组和14～15月龄组共3组,每组10只。用比色法测定大鼠皮质、海马和纹状体区ChAT与AChE的活性。结果与4～5月龄组大鼠和4～5周龄组大鼠大脑皮质、海马和纹状体区ChAT活性比较,14～15月龄组大鼠明显降低(均P<0.01);4～5月龄组与4～5周龄组大鼠大脑皮质、海马、纹状体区ChAT活性比较,均没有显著性差异(P>0.05);与4～5月龄组和4～5周龄组比较,14～15月龄组大鼠3个脑区AChE活性明显降低(分别为P<0.05,P<0.01);4～5月龄组与4～5周龄组大鼠3个脑区AChE活性比较,均没有显著性差异(P>0.05)。结论年龄与胆碱能神经系统中ChAT和AChE的活性呈负相关。更多还原

【Abstract】 Objective To investigate the effect of age on activity of cholineacetyltransferase(ChAT) and acetylcholinesterase(AChE) in cerebral cholinergic nerve system of rat.Method According to age,male Sprague-Dawley rats were divided into the following 3 groups at random: 4～5 weeks old group,4～5 months old group,14～15 months old group(n=10,respectively).Chromatometry was used to assay the activity of ChAT and AChE in cerebral cortex,hippocampus and corpus striatum.Results In 14～15 months old rats,activity of ChAT and AChE in cerebral cortex,hippocampus and corpus striatum was prominantly reduced,as compared with that in 4～5 months old rats and in 4～5 weeks old rats(ChAT: P<0.01;AChE: P<0.05 and P<0.01 respectively);However,activity of ChAT and AChE was not remarkly different between 4～5 weeks old rats and 4～5 months old rats(ChAT and AChE: P>0.05).Conclusion There is a negative correlation between age and activity of ChAT and AChE in cerebral cholinergic nerve system.更多还原