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冷活性纤维素酶性质研究

Characteristics of the cold-active cellulase

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【作者】 王玢汪天虹

【Author】 WANG Bin, WANG Tian-hong(State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China)

【机构】 山东大学微生物技术国家重点实验室山东大学微生物技术国家重点实验室 山东济南250100山东济南250100

【摘要】 对海洋嗜冷菌MB1所产冷活性纤维素酶的性质进行了研究。该酶最适反应温度为35℃ ,0℃时残余酶活约为20% ,最适 pH值为6.0,在pH5~7.5范围内酶活均较高。该酶对热敏感 ,50℃保温2h ,残余酶活为15 % ;60℃时 ,酶完全失活。

【Abstract】 In this papger properties of cold-active cellulase from marine cold-adaptive bacteria MB1 were characterized. Major characteristics found were that the enzyme has a optimum temperature of 35 ℃ and retained about 20% of maximal activity at 0 ℃. Optimum pH was 6.0 with cellulase active between pH 4.5~7.6. Low thermal stability was evident with the enzyme maintaining about 15% of its maximum activity after 2 h of exposure and ceasing activity after heat treatment at 60 ℃.

  • 【文献出处】 海洋科学 ,Marine Sciences , 编辑部邮箱 ,2004年02期
  • 【分类号】Q936
  • 【被引频次】13
  • 【下载频次】179
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