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雌二醇中残留溶剂测定方法的建立
Establishment of a Method for Determination of Residual Solvent in Estradiol
【摘要】 建立顶空气相色谱法,同时测定雌二醇中的残留溶剂甲醇和乙醇。采用以6%氰丙基和94%二甲基聚硅氧烷为固定相的毛细管柱作为色谱柱,用氢火焰离子化检测器检测。方法学验证结果表明:甲醇质量浓度为2.01~20.1μg/m L时残留溶剂线性关系良好(r=0.999 8),乙醇质量浓度为2.00~24.0μg/m L时残留溶剂线性关系良好(r=0.999 8);甲醇回收率分别为106.9%、102.2%、100.8%,乙醇回收率为104.3%、103.8%、105.9%。该方法专属性强、灵敏度高、准确性好,可用于雌二醇中残留溶剂的测定。
【Abstract】 The paper established a headspace gas chromatography method and simultaneously determined the residual solvents methanol and ethanol in estradiol. The paper uses a capillary column with 6% cyanopropyl and 94% dimethylpolysiloxane as stationary phases as the chromatographic column, and is detected by a hydrogen flame ionization detector. The methodological validation results indicate that methanol ranges from 2.01 to 20.1 μ. The linear relationship is good within the range of g/mL(r=0.999 8), and ethanol ranges from 2.00 to 24.0 μ. The linear relationship is good within the range of g/mL(r=0.999 8); The recovery rates of methanol are 106.9%, 102.2%, and 100.8%, respectively, while the recovery rates of ethanol are 104.3%, 103.8%, and105.9%. This method has strong specificity, high sensitivity, and good accuracy, and can be used for the determination of residual solvents in estradiol.
- 【文献出处】 工业微生物 ,Industrial Microbiology , 编辑部邮箱 ,2024年02期
- 【分类号】O657.7;TQ460.72
- 【下载频次】91