【摘要】 目的:优化液相色谱法测定茶枯粉中三种防腐剂含量的分析方法。方法:以山梨酸、苯甲酸与糖精钠为标准物质,Syncronis C18(250×4.6 mm,5μm)为分离柱,测定波长为254 nm,3%甲醇与97%20 mmo/L的乙酸铵溶液为流动相。茶枯粉经过正己烷破脂,蛋白剂沉淀的测定条件下,以外标法定量。结果防腐剂的加标回收率在95%～108%之间,方法检出限在3.87～4.39 mg/kg之间。结论:该方法操作过程简单,结果重现性好,灵密度高,适用于茶枯粉中三种防腐剂含量的测定。更多还原

【Abstract】 objective to optimize the analytical method for the determination of three preservatives in tea seed powder. Methods sorbic acid, benzoic acid and saccharin sodium were used as reference materials, Syncronis C18(250×4.6 mm, 5 μm) was used as separation column, the wavelength was 254 nm, 3 % methanol and 97 % 20 mmol/L ammonium acetate solution were used as mobile phase. Tea seed powder after n-hexane break fat, protein agent precipitation of the determination of the conditions, the external standard method quantitative. The results showed that the recovery of preservatives was between 95 % and 108 %, and the detection limit was between 3.87 mg/kg and 4.39 mg/kg. Conclusion: The method is simple, reproducible and suitable for the determination of three preservatives in tea seed powder.更多还原