【摘要】 采用超声辅助水提醇沉法提取白蜡多年卧孔菌(Perenniporia fraxinea)子实体与菌丝体多糖,利用单因素试验结合Box-Behnken响应面法优化子实体与菌丝体多糖提取工艺参数,并测定其DPPH·、ABTS+·清除能力。结果表明,子实体多糖的最佳提取工艺条件为:料液比1∶60(g∶m L)、提取时间76 min和超声时间16 min,在此优化条件下,子实体多糖提取率为4.39%;菌丝体多糖的最佳提取工艺条件为:料液比1∶30(g∶m L)、提取时间101 min和超声时间16 min,在此优化条件下,菌丝体多糖提取率为6.33%。当子实体和菌丝体多糖质量浓度为2.0 mg/m L时,子实体对DPPH·、ABTS+·清除率分别为45.67%和72.89%,菌丝体多糖对DPPH·、ABTS+·的清除率分别为51.67%和75.83%。子实体和菌丝体多糖能降低植物油过氧化值(POV),表明白蜡多年卧孔菌多糖具有一定的抗油脂氧化的能力。更多还原

【Abstract】 Polysaccharides in fruiting bodies and mycelium of Perenniporia fraxinea were extracted by ultrasonic-assisted water extraction and alcohol precipitation method. The extraction process conditions of polysaccharides in fruiting bodies and mycelium were optimized by single factor experiments and Box-Behnken response surface method, and the DPPH·, ABTS+·scavenging ability were determined. The results showed that the optimal extraction conditions of fruiting bodies polysaccharides were as follows: solid to liquid ratio 1∶60(g∶ml), extraction time 76 min, ultrasonic time 16 min. Under these optimized conditions, the extraction rate of fruiting bodies polysaccharides was 4.39%. The optimal extraction conditions of mycelium polysaccharides were as follows:solid and liquid ratio 1∶30(g∶ml), extraction time 101 min, ultrasonic time 16 min. Under these optimized conditions, extraction rate of mycelium polysaccharides was 6.33%. When the mass concentration of fruiting bodies polysaccharides and mycelium polysaccharides was 2.0 mg/ml, the scavenging rates fruiting bodies polysaccharides on DPPH·and ABTS+·were 45.67%, 72.89%, and the scavenging rates of mycelium polysaccharides on DPPH·and ABTS+·were 51.67%, 75.83%, respectively. Fruiting bodies and mycelium polysaccharides could reduce the peroxide value(POV) of vegetable oil, which showed that P. fraxinea had certain anti-lipid oxidation ability.更多还原