【摘要】 目的:探讨针灸改善骨髓抑制,促进白细胞升高的分子生物学机制。方法:选用清洁级、雄性昆明种小鼠224只,随机分为正常组、模型组、针刺组、艾灸组,每组56只。用环磷酰胺(CTX)造成骨髓抑制模型。针刺组、艾灸组选取"大椎""膈俞""肾俞""足三里",分别进行针刺、艾灸,正常组、模型组每日与针刺组和艾灸组同时抓取、固定,不做任何治疗。各组分别于第2～7天用免疫组化法观察骨髓细胞DNA聚合酶β(polβ)、切除修复交叉互补基因(XPD)表达的动态变化。结果:针刺和艾灸可以明显上调CTX模型小鼠骨髓细胞DNA修复蛋白XPD、polβ的表达,促进骨髓细胞DNA损伤的碱基切除修复和核苷酸切除修复,从而减轻烷化剂CTX造成的骨髓抑制,增加白细胞。结论:促进骨髓细胞DNA的切除修复,保护造血细胞因化学药物引起的细胞损伤,是针灸改善化疗后骨髓抑制,保护造血功能,提升白细胞的重要机制之一。更多还原

【Abstract】 Objective To explore the molecular biological mechanism of acupuncture and moxibustion for relieving myelosuppression and increasing white blood cells. Methods Two hundred and twenty-four clean male Kunming mice were randomly divided into a control group,a model group,an acupuncture group and a moxibustion group,56 mice in each group. The model of myelosuppression was made with Cyclophosphamide. In the acupuncture group and the moxibustion group,acupoints "Dazhui" (GV 14),"Geshu" (BL 17),"Shenshu" (BL 23) and "Zusanli" (ST 36) were used for treatment with acupuncture and moxibustion,respectively,while,in the control group and the model group,there were no treatment carried out except catching and fixing. The changes of bone marrow cell DNA pol β and XPD between the 2nd and 7th day were examined with immunohistochemical method. Results Acupuncture and moxibustion markedly up-regulated the expression of bone marrow cell DNA pol β and XPD,and promoted the base excision repair and nucleotide excision repair,which leads to the relieving Cyclophosphamide-induced myelosuppression and increasing the number of white blood cells. Conclusion For acupuncture and moxibustion,one of the bone major mechanisms in relieving post-chemotherapy myelosuppression,protecting hemopoietic function and increasing the white blood cells is that it can promote the repair of the bone marrow cell DNA excision and protect hemopoietic cells from injury by chemical drugs.更多还原