【摘要】 目的 观察玉竹提取物（POD）对多囊卵巢综合症（PCOS）模型大鼠卵巢功能及炎症的影响。方法 本研究选择3周龄雌性SD大鼠，采用来曲唑进行灌胃21 d复制PCOS模型，造模成功后，采用不同浓度POD对PCOS模型大鼠进行灌胃处理。记录大鼠每日饮水及摄食量、体重变化情况及动情周期，检测血液相关指标、测定血清睾酮水平，检测大鼠糖耐量，采用苏木素-伊红（HE）染色观察卵巢组织形态学变化，通过蛋白质免疫印迹法（WB）、实时荧光定量反转录聚合酶链反应（qRT-PCR）及免疫组织化学法（IHC）检测卵巢组织内抗苗勒激素（AMH）、炎性细胞因子白介素-1β (IL-1β)、肿瘤坏死因子α (TNF-α)的表达。结果 与对照组相比，PCOS模型大鼠动情周期紊乱、卵巢呈多囊样改变，血清睾酮水平显著上升、卵巢AMH表达上调、糖耐量异常（P<0.01）,IL-1β和TNF-α表达增加（P<0.01）；不同浓度POD处理PCOS模型大鼠后，大鼠动情周期逐渐恢复正常、卵巢中黄体数量增加、囊性卵泡数量减少、血清睾酮水平下调、糖耐量异常缓解（P<0.01），卵巢组织炎性细胞因子IL-1β、TNF-α表达下调。结论 POD可以改善PCOS模型大鼠卵巢功能并且抑制炎症反应，对模型大鼠的PCOS有一定的治疗效果。更多还原

【Abstract】 Objective To investigate the effects of Polygonatum odoratum(Mill.) Druce extract(POD) on ovarian function and inflammation of rats with polycystic ovary syndrome(PCOS). Methods Three-week-old female SD rats were used in this study. PCOS model was established by gavage of letrozole for 21 d. Then PCOS model rats were treated with different concentrations of POD by gavage. The changes of water and food intake,body mass and estrous cycle were recorded, blood related indicators were detected, serum testosterone level was measured, glucose tolerance was detected, and the morphological changes of ovarian tissue were observed by HE staining. Western blot(WB), fluorescent quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) and immunohistochemistry were used to detect the expression of anti-Mullerian hormone(AMH),inflammatory cytokines interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in ovarian tissues.Results Compared with the control group, the PCOS model rats had disordered estrous cycle, polycystic ovarian changes, significantly increased serum testosterone level, up-regulated ovarian AMH expression, and impaired glucose tolerance(P<0.01). The expression of IL-1β and TNF-α was increased(P<0.01). After the PCOS model rats were treated with different concentrations of POD, the estrous cycle gradually returned to normal, the number of corpus luteum in the ovary increased, the number of cystic follicles decreased, the serum testosterone level decreased, and the impaired glucose tolerance was alleviated(P<0.01). The expression of inflammatory cytokines IL-1β and TNF-α in ovarian tissue was down-regulated. Conclusion POD can improve the ovarian function of PCOS model rats and inhibit inflammatory response, and has a certain therapeutic effect on PCOS model rats.更多还原