【摘要】 目的：探讨P53介导的miR-30e-5p对乳腺癌细胞恶性表型的抑制作用。方法：利用染色质免疫共沉淀试验与荧光定量PCR验证P53对miR-30e-5p的转录激活作用；分别采用CCK8试验、划痕试验和Transwell小室试验检测miR-30e-5p对于乳腺癌细胞增殖、迁移和侵袭能力的影响；通过激光共聚焦技术探究miR-30e-5p对β-catenin亚细胞定位的影响，并利用Western blot法检测β-catenin及其靶蛋白的表达情况。结果：P53可与MCF-7、MDAMB-453细胞的miR-30e-5p启动子区域结合，转录激活miR-30e-5p的表达（P <0.05）；乳腺癌细胞转染miR-30e-5p后，其增殖、迁移、侵袭能力受到不同程度抑制（P <0.05）；此外，miR-30e-5p可减少β-catenin的核内分布，抑制β-catenin靶基因表达。结论：P53可介导抑癌基因miR-30e-5p表达，通过抑制经典Wnt通路而抑制乳腺癌细胞恶性表型。更多还原

【Abstract】 Objective: To explore the inhibitory effect of p53-mediated miR-30e-5p on malignant phenotype of breast cancer cells. Methods: Chromatin immunoprecipitation and quantitative PCR were used to verify the transcriptional activation effect of P53 on miR-30e-5p. The effects of miR-30e-5p on the proliferation, migration and invasion of breast cancer cells were detected by CCK8, wound healing and Transwell assays, respectively. Confocal laser was used to explore the effect of miR-30e-5p on the subcellular localization of β-catenin. Western blot was used to detect the expressions of β-catenin and its target proteins. Results:P53 can bind to the miR-30e-5p promoter region of MCF-7 and MDA-MB-453 cells, and transcriptionally activates the expression of miR-30e-5p( P < 0.05). The breast cancer cells’ proliferation, migration and invasion abilities were inhibited to varying degrees after they were transfected with miR-30e-5p( P < 0.05). In addition, miR-30e-5p can reduce the distribution of β-catenin in nuclei and inhibit the expression of target genes of β-catenin. Conclusion : P53 can mediate the expression of miR-30e-5p, a tumor suppressor gene, and inhibit the malignant phenotypes of breast cancer cells by mediating the canonical Wnt pathway.更多还原