【摘要】 目的探讨社区人群血管紧张素转换酶2(ACE2)基因G8790A多态性与原发性高血压的相关性。方法以青岛市四个社区中筛检出的原发性高血压患者236例(病例组)及血压正常者241例(对照组)为研究对象,应用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)鉴定ACE2基因G8790A多态性,并采用多因素Logis-tic回归分析控制混杂因素的影响。结果 ACE2基因G8790A等位基因G在病例组与对照组的频率分别为,男性:71.30%、58.82%,两组比较差异无统计学意义(χ2=3.288,P=0.070);女性:55.86%、45.19%,两组比较差异有统计学意义(χ2=6.400,P=0.011)。基因型AA、AG和GG在女性病例组的频率分别是19.53%、49.22%、31.25%,对照组分别是33.33%、42.95%、23.72%,两组基因型分布比较,差异有统计学意义(χ2=7.015,P=0.030)。相对于AA基因型,AG和GG基因型的居民罹患原发性高血压的危险性均增加(OR=1.99,P=0.022;OR=2.25,P=0.015)。控制年龄、超重、吸烟、饮酒、血脂等混杂因素后,男性:与等位基因A相比,携带等位基因G的居民罹患原发性高血压的危险性增加(OR=2.09,P=0.038);女性:与AA基因型相比,GG基因型的居民罹患原发性高血压的危险性增加(OR=2.69,P=0.015)。结论 ACE2基因G8790A变异与原发性高血压存在相关性,男性携带G等位基因和女性携带GG基因型可能使原发性高血压发生的危险性增加。更多还原

【Abstract】 Objective To investigate the association of angiotensin-converting enzyme 2(ACE2) gene G8790A polymorphism with essential hypertension(EH) in community populations.Methods Totally 236 patients screened from four communities of Qingdao and 241 healthy subjects were enrolled in this study.The polymorphisms of G8790A in ACE2 gene were detected with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Multivariate logistic regression analysis was performed to adjust the effects of confounding factors.Results The frequency of G allele of ACE2 gene was 71.30% in EH group and 58.82% in control group in male subjects respectively(χ2=3.288,P=0.070),and 55.86% in EH group and 45.19% in control group in female subjects(χ2=6.400,P=0.011)respectively.For the females,the frequencies of genetypes were as follows:AA 19.53%,AG 49.22%,and GG 31.25% in EH group,and AA 33.33%,AG 49.22%,and GG 23.72% in control group(χ2=7.015,P=0.030).The subjects with AG or GG genotype had significantly higher risk for EH than that with AA genotype(OR=1.99,P=0.022;OR=2.25,P=0.015).After having been adjusted by logistic regression for age,body mass index(BMI),smoking,and alcohol use,total cholesterol,and triglyceride,the male subjects with G allele had significantly higher risk for EH than those with allele A(OR=2.09,P=0.038),and the female subjects with GG genotype had significantly higher risk for EH than those with AA genotypes(OR=2.69,P=0.015).Conclusion The ACE2 gene G8790A polymorphism may be associated with essential hypertension.Male populations with G allele and female populations with GG genotype may have higher risk of essential hypertension.更多还原