【摘要】 建立了同时检测蔬菜水果中百菌清及其代谢物4-羟基百菌清的高效液相色谱-串联质谱分析方法。样品经酸化乙腈提取后，采用配备大气压化学电离源的三重四极杆串联质谱仪测定。结果表明：百菌清及其代谢物4-羟基百菌清在0.005～0.2 mg/L范围内峰面积与质量浓度的线性关系良好，决定系数（R2）大于0.99；方法定量限为0.01 mg/kg。在0.01、0.05和0.2 mg/kg3个添加水平下，百菌清及其代谢物4-羟基百菌清在结球甘蓝、黄瓜、葡萄和苹果4种基质空白样品中的平均回收率为83%～104%，相对标准偏差（RSD）为0.73%～7.7%(n=6)。该方法简单快速、准确可靠，适合蔬菜水果中百菌清及其代谢物4-羟基百菌清的定量测定。更多还原

【Abstract】 An analytical method for simultaneous determination of chlorothalonil and its metabolite chlorothalonil-4-hydroxy in fruits and vegetables was developed using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS). The samples were extracted by acidified acetonitrile, and the extraction solution was analyzed by HPLC-MS/MS equipped with APCI ion source. The results showed that good linearity was obtained in the range of 0.005-0.2 mg/L with correlation coefficients(R2) greater than 0.99. The limits of quantification were 0.01 mg/kg. At the spiked levels of 0.01, 0.05, and 0.2 mg/kg, the average recoveries in four sample matrices(cabbage,cucumber, grape, and apple) were 83%-104% with the relative standard deviations(RSDs) varying from0.73% to 7.7%(n=6). The proposed method is simple, rapid, accurate and reliable, and it is suitable for the quantitative determination of chlorothalonil and its metabolite in fruits and vegetables.更多还原