【摘要】 采用Illumina MiSeq二代测序方法筛选得到青蛤丝裂原活化蛋白激酶(MAPK/p38)基因相似序列,进一步克隆得到全长cDNA.利用荧光定量PCR技术检测该基因在青蛤各组织中以及在鳗弧菌胁迫下的组织表达特征.结果显示,该基因全长共1781bp,开放阅读框长度为1104bp,编码367个氨基酸.在正常条件下该基因在青蛤体各组织中都表达,其中血淋巴中表达量最高,肝脏次之,鳃中最少.经菌液侵染后12h青蛤血淋巴中表达量即达到峰值,随后表达量逐渐降为正常水平.更多还原

【Abstract】 MAPK/p38likely-sequence was obtained by Illumina Miseq sequencing and the fulllength cDNA was cloned.The gene expressions were detected in different tissues in normal condition and vibrio anguillarum stress condition by RT-qPCR.The results showed that,the full length of MAPK/p38 gene was 1781 bp,while the ORF was 1104 bp which encodes 367 amino acid.In normal condition,MAPK/p38 expressed in all tissues.The expression level was high in hemolymph and low in cheek.While under vibrio anguillarum infection condition,MAPK/p38 expression reached the peak at 12 hafter infection,and then reduced to normal level.更多还原