【摘要】 以大分子有机物为碳源，考察短时顶部进水+厌氧搅拌(R1)与长时底部推流进水+厌氧静置(R2)两种序批式反应器对生物除磷污泥颗粒化的影响。结果表明：R1反应器污泥一直维持絮体状态且易发生丝状膨胀，SVI30在88.85～1212.12 mL·g-1之间波动；R2反应器污泥可在110d实现颗粒化，成熟颗粒污泥结构密实，SVI30为38.39～58.92mg·L-1。R2反应器在厌氧段挥发性脂肪酸产量更高，更有利于聚磷菌的生长。R2反应器污泥胞外聚合物(EPS)中蛋白质和多糖含量均高于R1，分别为26.24 mg·gVSS-1和7.15 mg·gVSS-1，可与更多Ca、Mg结合促进污泥颗粒化。16SrRNA基因高通量测序显示，R2反应器有利于水解发酵菌群norank＿f＿Chitinophagaceae(27.71%)、norank＿f＿Saprospiraceae(4.86%)和分泌EPS菌群Flavobacterium(9.38%)的生长，促进了聚磷菌的生长和污泥的颗粒化。更多还原

【Abstract】 Using the macromolecular organic matter as the carbon source, the influence of the configuration on the granulation of the biological phosphorus removal sludge was investigated in two sequencing batch reactors(SBR), which were operated with a fast influent step from the top followed by an anaerobic mixing phase(R1) and an anaerobic plug flow feeding from the bottom followed by an anaerobic reaction without mixing(R2), respectively. The results indicated that the sludge in the R1 reactor maintained the floc state all the time and was easy to be filamentous bulking, and the SVI30 value was between 88.85 mL·g-1 and 1212.12 mL·g-1.The sludge in the R2 reactor could granulated around 110 d, and the mature granular sludge had a dense structure with the SVI30 value ranged from 38.39 mL·g-1 to 58.92 mL·g-1. The content of the volatile fatty acids(VFAs) in the R2 system was higher, which was beneficial for the growth of the phosphorus removing bacteria.The extracellular polymers(EPS) of the sludge in the R2 reactor were higher than that in the R1 reactor, which were 26.24 and 7.15 mg·gVSS-1 respectively and could combine with more Ca and Mg to promote the sludge granulation. The 16 S rRNA gene high throughput sequencing analysis showed that the R2 reactor promoted the growth of the norank＿f＿Chitinophagaceae(27.71 %), norank＿f＿Saprospiraceae(4.86 %)which could hydrolyze and ferment the macromolecular organic matter and the Flavobacterium(9.38 %) secreting EPS., which were conducive to the growth of phosphorus removal bacteria and the sludge granulation.更多还原