【摘要】 目的：研究云南蓍Achillea wilsoniana Heimerl ex Hand.-Mazz.的黄酮类化学成分及其体外抗炎活性。方法：采用多种色谱技术对云南蓍70%乙醇提取物进行分离纯化，并根据化合物的理化性质与波谱数据进行结构鉴定。采用脂多糖(LPS)诱导的小鼠巨噬细胞RAW 264.7体外炎症模型测定化合物对一氧化氮(NO)释放的抑制作用。结果：从云南蓍中分离得到22个黄酮类化合物，分别鉴定为：木犀草素(1)、hydnocarpin(2)、槲皮素(3)、异荭草素(4)、quercetin-3-O-α-arabinopyranosyl(1→6″)-β-glucopyranoside(5)、芦丁(6)、异鼠李素-3-O-β-D-芸香糖苷(7)、异金雀花素(8)、异鼠李素-3-O-α-L-吡喃阿拉伯糖(1→6)-β-D-吡喃葡萄糖苷(9)、异槲皮苷(10)、蒿黄素(11)、槲皮素-4′-O-β-D-葡萄糖苷(12)、异牡荆苷(13)、芹菜素-7-O-β-D-吡喃葡萄糖苷(14)、苜蓿素-7-O-β-D-吡喃葡萄糖苷(15)、异鼠李素-3-O-β-D-葡萄糖苷(16)、芹菜素(17)、异鼠李素(18)、casticin(19)、槲皮苷(20)、泽兰黄素(21)、vitexin(22)。体外抗炎实验结果表明，化合物1、3、11、13～15、17、19和21对LPS诱导的RAW 264.7细胞NO生成具有一定的抑制活性。结论：其中，化合物1、3～7、10、11、13～15、17～19、21、22为首次从云南蓍中分离得到，化合物2、8、9、12、16、20为首次从蓍属植物中分离得到，化合物1、3、11、13～15、17、19、21具有一定的抗炎活性。更多还原

【Abstract】 Objective: To study the chemical constituents of flavonoids from Achillea wilsoniana and anti-inflammatory activity in vitro.Methods: The 70% ethanol extract of Achillea wilsoniana was isolated and purified by various chromatographic techniques, the structures were identified on the basis of physicochemical properties, spectral data.The anti-inflammatory activities of the compounds were evaluated by detecting the release of inflammatory mediators NO from mice macrophages RAW264.7 induced by lipopolysaccharide(LPS).Results: Twenty-two compounds were isolated from Achillea wilsoniana and identified as luteolin(1),hydnocarpin(2),querceentin(3),isoorientin(4),quercetin-3-O-α-arabinopyranosyl(1→6″)-β-glucopyranoside(5),rutin(6),isorhamnetin-3-O-β-D-rutinoside(7),isoplantagenin(8),isorhamnetin-3-O-α-L-arabinose(1→6)-β-D-glucopyranoside(9),isoquercitrin(10),artemein(11),quercetin-4′-O-β-D-glucoside(12),isovitexin(13),apigenin-7-O-β-D-glucopyranoside(14),tricin-7-O-β-D-glucopyranoside(15),isorhamnetin-3-O-β-D-glucoside(16),apigenin(17),isorhamnetin(18),casticin(19),quercitrin(20),eupatrin(21),vitexin(22),respectively.The anti-inflammatory experiments in vitro showed that compounds 1,3,11,13 to 15,17,19 and 21 had certain inhibitory activities against LPS-induced NO production in RAW 264.7 cells.Conclusion: Among them, compounds 1,3 to 7,10,13 to 15,17 to 19,21,22 are isolated from Achillea wilsoniana for the first time, compounds 2,8,9,12,16,20 are isolated from Achillea genus for the first time.Compounds 1,3,11,13 to 15,17,19,21 show certain anti-inflammatory activity.更多还原