【摘要】 目的:构建光热型脂质膜包覆大肠杆菌的复合物，并对其性能进行考察。方法:通过薄膜分散法制备光热型脂质膜(LMI),利用静电吸附作用制备脂质膜包覆的大肠杆菌(LMI@Ec)。激光散射粒度仪分析LMI@Ec的粒径和电位；透射电子显微镜和激光共聚焦显微镜观察LMI包覆前后大肠杆菌形态；平板计数法和聚丙烯酰胺凝胶电泳分别检测LMI的包覆对大肠杆菌的活性及其蛋白表达谱的影响；红外光热成像仪检测LMI@Ec的光热效应。结果:制备的LMI@Ec粒径为(1 990.5±132) nm,电位为-(5.09±0.52) mV,透射电子显微镜图像显示涂层细菌上有一个清晰的外壳；脂质膜的包覆对大肠杆菌的生物活性几乎无影响；激光照射后细菌活性下降，但在LB培养基中进行温度梯度培养10 h后其生长曲线可恢复到与未涂层菌相当。LMI@Ec具有良好的光热性能，能够进行正常的蛋白表达。体外细胞毒性实验表明，LMI@Ec经激光照射后对B16F10和HepG2细胞均具有良好的杀伤作用。结论:LMI@Ec的成功制备为后续深入研究工程菌介导的疾病治疗提供了新的研究方向。更多还原

【Abstract】 Objective: To prepare photothermal lipid membrane coated Escherichia coli and study the properties. Methods: The photothermal lipid membrane(LMI) was prepared by thin film dispersion method, and the electrostatic adsorption method was used to coat Escherichia coli with the lipid membrane(LMI@Ec). The size and potential of LMI@Ec were analyzed by laser scattering particle size analyzer, the morphology of E.coli and LMI@Ec was observed by transmission electron microscopy and laser confocal microscopy, and plate counting method was used to detect cell viability of LMI@Ec. In addition, the effect of protein expression of LMI@Ec was evaluated by polyacrylamide gel electrophoresis, and the photothermal effect of LMI@Ec was detected by Infrared Photothermal Imager. Results: LMI@Ec had a smooth surface with the average particle size of(1 990.5±132) nm and the potential of-(5.09±0.52) mV. The coating of the lipid membrane had little effect on the activity of E.coli. Although the bacterial activity decreased after laser irradiation, the growth curve recovered to the same level as wild bacteria after 10 h of temperature gradient culture in LB medium. LMI@Ec had well photothermal properties and can normally express protein. In vitro cytotoxicity experiments showed that LMI@Ec under 808 nm laser irradiation presented enhanced antitumor effect on B16F10 and HepG2 cells. Conclusion:The successful preparation of LMI@Ec provides a new research direction for the subsequent in-depth study of bacteria-mediated disease treatment.更多还原