【摘要】 目的 探讨长链非编码RNA生长停滞特异性转录本5(LncRNA GAS5)对结直肠癌5-氟尿嘧啶(5-FU)耐药的作用。方法 选取人结肠癌细胞系HCT-8和SW480,建立5-FU耐药细胞株,构建LncRNA GAS5过表达结直肠癌细胞模型。反转录-聚合酶链式反应(RT-PCR)检测LncRNA GAS5在结直肠癌5-FU耐药细胞系中表达；CCK-8检测细胞增殖；流式细胞术检测细胞凋亡。结果 RT-PCR检测显示, LncRNA GAS5在结直肠癌HCT-8细胞与SW480细胞中的表达水平低于正常结肠细胞,差异有统计学意义(P<0.05);LV5-GAS5组HCT-8细胞、SW480细胞中LncRNA GAS5表达高于Vector组,差异有统计学意义(P<0.05)。CCK-8实验检测显示, LncRNA GAS5能明显抑制HCT-8细胞和SW480细胞的增殖(P<0.05)。AnnexinV/PI双重染色及流式细胞术结果显示,在结直肠癌HCT-8细胞与SW480细胞中LV5-GAS5组的细胞凋亡比例高于Vector组,差异有统计学意义(P<0.05)。结论 LncRNA GAS5在结直肠癌5-FU耐药中发挥重要作用,能抑制结肠癌细胞系的增殖,并促进细胞凋亡。更多还原

【Abstract】 Objective To discuss the role of long non-coding RNA-growth arrest-specific 5(LncRNA GAS5) on 5-fluorouracil(5-FU) resistance in colorectal cancer. Methods Human colon cancer cell lines HCT-8 and SW480 were selected to establish 5-FU resistant cell lines, and LncRNA GAS5 overexpression colorectal cancer cell model was constructed. LncRNA GAS5 expression was detected by reverse transcription polymerase chain reaction(RT-PCR) in 5-FU resistant cell lines of colorectal cancer. CCK-8 was used to detect cell proliferation, and apoptosis was detected by flow cytometry. Results RT-PCR showed that the expression level of LncRNA GAS5 in colorectal cancer HCT-8 and SW480 cells was significantly lower than that in normal colon cells, and the difference was statistically significant(P<0.05). The expression of LncRNA GAS5 in HCT-8cells and SW480 cells in LV5-GAS5 group was significantly higher than that in Vector group, and the difference was statistically significant(P<0.05). CCK-8 assay showed that LncRNA GAS5 could significantly inhibit the proliferation of HCT-8 cells and SW480 cells(P<0.05). AnnexinV/PI double staining and flow cytometry showed that the apoptosis ratio of LV5-GAS5 group in HCT-8 cells and SW480 cells was also significantly higher than that in vector group, and the difference was statistically significant(P<0.05). Conclusion LncRNA GAS5 plays an important role in the 5-FU resistance of colorectal cancer, which can inhibit the proliferation of colon cancer cell lines and promote cell apoptosis.更多还原