【摘要】 [目的]探讨不同剂量膳食钙对高脂膳食大鼠血糖、血脂、胰岛素及血清瘦素(leptin)水平的影响。[方法]将34只雄性SD大鼠随机分成5组,分别是A组,高脂普通钙组[脂肪34%(产热比),钙0.4%(w/w)];B组,高脂中钙组[脂肪34%(产热比),钙1.2%(w/w)];C组,高脂高钙组[脂肪34%(产热比),钙2.4%(w/w)];D组,高脂高钙加奶粉组[脂肪34%(产热比)其中9%由奶粉提供,25%由猪油提供,钙2.4%(w/w)]、E组,基础饲料组[基础饲料,脂肪11%(产热比),钙0.4%(w/w)],其中A、B、C、D组各有7只大鼠,E组有6只大鼠。每周周一9:00,周四21:00测体重。6周后断头取血测血糖、血脂和血清胰岛素含量,放射免疫分析法测血清中leptin的含量。[结果]与A组比较,C、D、E各组大鼠体重均显著降低(P﹤0.05);A组血清胆固醇显著高于D组(P﹤0.05);各组血清高密度脂蛋白、甘油三酯和血糖差异均无统计学意义(P﹥0.05);与A组比较C、D、E组血清胰岛素水平显著降低(P﹤0.05),其中C血清组胰岛素与A组比较差异有统计学意义(P﹤0.01);与E组比较A、B组血清leptin水平明显升高(P﹤0.05)。[结论]高膳食钙降低了大鼠体重、血清总胆固醇水平,改善了高胰岛素血症,其作用机理可能是钙通过影响脂肪组织中leptin的分泌水平,从而抑制了高脂膳食诱导的肥胖。更多还原

【Abstract】 [Objective]To explore the effects of dietary calcium on the blood lipid,insulin and leptin of rats fed a high fat diet [Methods]34 male SD rats were randomly divided into 5 groups,A group was fed a high fat and normal calcium diet(34% energy from fat,0.4%calcium,w/w),B group was fed high fat and medium dosage calcium diet(34% energy from fat,1.2%calcium,w/w),C group was fed high fat and high calcium diet(34% energy from fat,2.4%calcium,w/w),D group was fed high fat and high calcium diet(34% energy from fat,9% from dairy product,2.4% calcium,w/w),E group was fed basic diet(11% energy from fat,0.4%calcium).Each group contained 6-7 animals.After 6 weeks,blood glucose,blood lipid,serum insulin and leptin were measured.[Results]Compared with those of A group,the body weight of C,D,E groups decreased significantly(P﹤0.05),The serum cholesterol in A group increased and compared with that of A group,the serum cholesterol in D group decreased significantly(P﹤0.05),but there were no significant difference among all the groups in high density lipoprotein,triglyceride and the blood glucose(P﹥0.05).Compared with that of A group,the serum insulin in C,D,E groups decreased significantly(P﹤0.05),and compared with that of A group,the serum insulin in C group decreased extremely(P﹤0.01),the leptin in A and B groups,compared with that of E group,increased significantly(P﹤0.05).[Conclusion]Dietary calcium can reduce serum cholesterol and affect the leptin,improve hyperinsulinemia,thereby inhibite the obesity induced by high fat diet.更多还原