【摘要】 目的 了解血管活性物质AngⅡ、NO对VEC、VSMC增殖及凋亡的影响。方法 在培养的VEC、VSMC细胞中加入NO、AngⅡ等物质,观察细胞生长及凋亡情况。结果 NO诱导VSMC凋亡而AngⅡ抑制VSMC凋亡,NO拮抗AngⅡ对VEC凋亡的诱导作用。结论 血管活性物质NO、AngⅡ调节VEC及VSMC增殖与凋亡的能力与调节血管张力的相互抵消作用一致,这些血管活性物质的表达失去平衡可使调节血管张力、细胞增殖及细胞死亡的内环境稳定发生紊乱。更多还原

【Abstract】 Objective To investigate the effects of vasoactive substances, such as angiotensin II and nitric oxide (NO) ,on the growth and apoptosis of vascular smooth muscle cells and endothelium cells. Methods To observe the cells growth and apoptosis conditions, we uesd the NO and angiotensin II substances in cultured VEC and VSMC. Results The results showed that NO induced apoptosis and angiotensin I inhibited apoptosis in vascular smooth muscle cells. Whereas NOdirectly antagonized angiotensin I which induced apoptosis. Conclusion These findings suggested that the growth and apoptosis of vascular smooth muscle cells or endothelium cells were regulated by the antagonistic balance between vasoactive substances such as NO and angiotensin II . The countervailing balance between NO and angiotensin H may determing the overall cell population within the vessel wall by regulating genetic programs deteming cell death as well as cell growth.更多还原