【摘要】 ‘巨峰’葡萄果实采后活性氧（reactive oxygen species,ROS）代谢旺盛，导致其贮藏期缩短，商业价值降低。本研究使用不同质量浓度（0（对照）、10、30、50、70 mg/L）胺鲜酯（diethyl aminoethyl hexanoate,DA-6）对转色期葡萄进行喷施处理，探讨DA-6处理对采后‘巨峰’葡萄果实品质和ROS代谢的影响。通过测定与品质相关的生理指标，发现DA-6处理明显降低了葡萄果实质量损失，并延缓了葡萄果实硬度、可溶性固形物含量的下降，其中质量浓度50 mg/L DA-6处理效果最佳；与对照组相比，50 mg/L DA-6处理可明显提升果实中超氧化物歧化酶、抗坏血酸过氧化物酶、过氧化氢酶、谷胱甘肽还原酶活力以及1,1-二苯基-2-三硝基苯肼自由基清除率和总还原能力，同时也能够抑制还原型抗坏血酸、还原型谷胱甘肽含量下降，降低超阴氧离子自由基产生速率和丙二醛含量。综上所述，采前喷施DA-6维持了采后葡萄果实ROS清除系统的稳定性，将ROS代谢维持在一个相对平衡的状态，减轻了氧化损伤，延缓了果实衰老，延长了‘巨峰’葡萄果实的货架期。更多还原

【Abstract】 The vigorous metabolism of reactive oxygen species(ROS) in postharvest ‘Kyoho’ grapes shortens the storage period, and reduce the commercial value. In this study, different concentrations(0, 10, 30, 50 and 70 mg/L) of diethyl aminoethyl hexanoate(DA-6) were sprayed on table grapes at veraison to investigate the effects of DA-6 treatment on postharvest fruit quality and ROS metabolism in ‘Kyoho’ grapes. By measuring the physiological indexes related to fruit quality, it was found that DA-6 treatment significantly reduced fruit mass loss, and delayed the decrease in fruit firmness and total soluble solid content, and this effect was most significant at a concentration of 50 mg/L. More importantly, 50 mg/L DA-6 treatment significantly increased the activity of superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging capacity and total reducing capacity, and suppressed the decrease in reduced ascorbic acid and glutathione contents and the accumulation of superoxide anion radical and malondialdehyde. In conclusion, pre-harvest spray of DA-6 maintained the stability of the ROS scavenging system in postharvest table grapes, kept ROS metabolism in a relatively balanced state, reduced oxidative damage, delayed fruit senescence, and prolonged the shelf life of ‘Kyoho’ grapes.更多还原