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油菜花粉多糖提取工艺条件研究

Polysaccharide Extraction Study on Rape Pollen

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【作者】 杨晓萍罗祖友吴谋成

【Author】 YANG Xiao-ping1,LUO Zu-you2,WU Mou-cheng2(1.College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China;2.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

【机构】 华中农业大学园艺林学学院华中农业大学食品科技学院华中农业大学食品科技学院 湖北武汉430070湖北武汉430070湖北武汉430070

【摘要】 对油菜花粉多糖的提取工艺进行研究。探讨浸提次数、时间、料液比和温度对多糖得率的影响,在单因素试验的基础上,通过正交试验确定最佳提取工艺条件。结果表明:温度对多糖得率的影响最大,其次为提取时间、次数,料液比的影响最小。油菜花粉多糖提取的最优条件为1:4的料液比,90℃水浴条件下,浸提4次,每次浸提4h,其水溶性多糖提取率达1.450%。

【Abstract】 The extraction of polysaccharides from rape pollen was studied. The effects of four factors such as times of extraction,time, ratio of solid-liquid and temperature were investigated. The results showed that the effect of temperature was the mostimportant, time was the second, times was the third, and the ratio of solid-liquid was the least. Through orthogonal test, theoptimum conditions of extraction were obtained on the basis of single factor test, as follows: ratio of solid-liquid 1:4, 90℃ for3h and 4 times extraction. The extraction percent of soluble polysaccharides was 1.450% on the optimum conditions.

【关键词】 油菜花粉多糖提取
【Key words】 rape pollenpolysaccharideextraction
【基金】 湖北省十五重点科技攻关计划(2001AA204A03)
  • 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2004年09期
  • 【分类号】TQ28
  • 【被引频次】38
  • 【下载频次】474
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