【摘要】 为提高竹笋加工废弃物的饲用价值，扩大动物饲料原料来源，本试验采用平板分离法从腐烂稻草中分离1株能降解纤维素的菌株HYH-1。通过形态学观察、生理生化试验和16S r DNA序列分析对分离菌株进行鉴定。以纤维素降解率为指标，通过单因素试验探究了葡萄糖添加量、KH₂PO₄添加量、Mg SO₄·7H₂O添加量、固水比、接种量、发酵时间、发酵温度等对纤维素降解率的影响。在单因素试验结果基础上，采用响应面法对纤维素降解率影响较大的因素如葡萄糖添加量、KH₂PO₄添加量、固水比和接种量进行了优化。结果表明：纤维素降解菌HYH-1被鉴定为耐盐芽孢杆菌（Bacillus halodurans）。建立了竹笋加工废弃物固体发酵条件的二次回归模型，该模型极显著，拟合度良好。适宜的发酵工艺为葡萄糖2.9%,KH₂PO₄2.9%,Mg SO₄·7H₂O 0.15%，固水比1:1，接种量11.50%，发酵时间8 d，发酵温度37℃。此条件下，竹笋加工废弃物中纤维素降解率达到54.85%。研究结果为竹笋加工废弃物的资源化利用提供了理论参考依据。更多还原

【Abstract】 To improve the feeding value of bamboo shoots processing waste and to expand the source of animal feed materials.A cellulose degrading strain HYH-1 was isolated from decaying rice straw by plate isolation method,and the isolate was identified by morphological observation,physiological and biochemical tests and 16S r DNA sequence analysis.The effects of glucose addition,KH₂PO₄addition,Mg SO₄·7H₂O addition,solid water ratio,inoculum amount,fermentation time and fermentation temperature on the cellulose degradation rate were investigated by single-factor test using cellulose degradation rate as the index.Based on the results of the single-factor test,the factors that had a significant effect on the cellulose degradation rate,such as glucose addition,KH₂PO₄addition,solid water ratio and inoculum,were optimized using the response surface method.The results showed that the cellulose degrading bacteria HYH-1 was identified as salttolerant Bacillus halodurans.A quadratic regression model for the solid fermentation conditions of bamboo shoot processing waste was established,which was highly significant with good fit.The suitable fermentation process was 2.9%glucose,2.9%KH₂PO₄,0.15%Mg SO₄·7H₂O,1:1 solid to water ratio,11.50%inoculum,8 d fermentation time and 37℃.Under these conditions,the cellulose degradation rate in bamboo shoot processing waste reached 54.85%.The results of the study provide a theoretical reference for the resource utilization of bamboo shoots processing waste.更多还原