【摘要】 为获得油菜抗菌核病的抗源材料，进行了油菜抗菌核病突变体筛选方法的研究．用油菜种子未成熟胚或成熟胚在ＭＳ－１（ＭＳ＋２，４－Ｄ２ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ＋ＫＴ０．１ｍｇ／Ｌ）培养基上诱导油菜愈伤组织，继代４～５次后用含草酸６００ｍｇ／Ｌ的无Ｃａ２＋ＭＳ－１培养基处理２～４ｍｍ大小的愈伤组织９６ｈ，筛选获得的耐毒素愈伤组织移至ＭＳ－２（ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ）培养基上获得再生小植株．更多还原

【Abstract】 The procedure of screening for rapeseed mutants with resistance to stem rot (Sclerotinia sclerotiorum (Lib) De Bary) was proposed.Rapeseed calli were induced from immature or mature embryos on the solid MS medium supplied with 2,4D 2 mg/L,IAA 0.2 mg/L and KT 0.1 mg/L. After subcultured for 4 or 5 generations on the same medium as above the calli were cut into segments in size about 2～4 mm and treated with Ca2+free oxalic acid at the concentration of 600 mg/L for 96 h. The selected resistant calli were transplanted onto the solid MS media supplied with BA 1 mg/L and IAA 0.2 mg/L to regenerate plantlets. Some resistant rapeseed variants were obtained.更多还原