【摘要】 目的 探讨橘皮提取物（5-ATAN）对多发性骨髓瘤细胞U266增殖和凋亡的影响及其机制。方法 用6.25μmol/L、12.5μmol/L、25μmol/L、50μmol/L、100μmol/L的5-ATAN处理对数生长期U266细胞24h，四甲基偶氮唑蓝（MTT）法检测细胞增殖抑制率，流式细胞术检测细胞凋亡率和线粒体膜电位，Western blotting法检测细胞色素C、BCL-2、BAX、pro-caspase 3、pro-caspase 9及cleaved-caspase 3、cleaved-caspase 9、PARP、Bad、p-Bad的表达。结果 5-ATAN对U266细胞的增殖有抑制作用，呈剂量依赖性（P<0.05）；细胞凋亡率随药物浓度的增加显著增加（P<0.05），线粒体膜电位随5-ATAN浓度的增加显著降低（P<0.05）。抗凋亡蛋白Bcl-2、p-Bad及pro-caspase 3、pro-caspase 9及PARP的表达逐渐降低；细胞色素C及促凋亡蛋白Bax、cleaved-caspase 3、cleaved-caspase 9、PARP(89KDa)的表达逐渐升高（P<0.05）。结论 5-ATAN抑制多发性骨髓瘤的增殖，促进其凋亡，可能与线粒体凋亡信号通路的激活有关。更多还原

【Abstract】 Objective To explore whether 5-ATAN could induce U266 cell lines apoptosis. Methods Use U266 which is a type of multiple myeloma cell lines as the research object. MTT assay was used to detect the antigrowth of 5-ATAN. Apoptosis and mitochondrial membrane potential(MMP)were detected by flow-cytometry. The expression levels of BCL-2,BAX,pro-caspase 3,pro-caspase 9 及 cleaved-caspase 3,cleaved-caspase 9,PARP,Bad,p-Bad were detected by western blotting. Results 1.5-ATAN can suppress the growth of U266 cell at a dose dependent manner,the highest inhibition ratio was 80.6% which was significantly higher than the control group. The apoptotic rate in the 5-ATAN groups was higher than the control group(P<0.05). The mitochondrial membrane potential is decreased with the increase concentration of 5-ATAN. The expression levels of Bcl-2,p-Bad,pro-caspase 3,pro-caspase 9 and PARP were decreased,while the cytochrome C,Bax,cleaved-caspase 3,cleaved-caspase 9,PARP(89KDa)were increased(P<0.05). Conclusion Treatment with 5-ATAN promoted apoptosis in U266 cells and inhibited their proliferation by activating the mitochondrial apoptosis signal pathway.更多还原