【摘要】 目的:对连翘(Forsythia suspensa (Thunb.) Vahl.)的化学成分进行研究。方法:利用反复硅胶和凝胶 LH-20, ODS柱层析进行分离和纯化, 通过理化性质和波谱数据分析鉴定化合物结构。结果:分离并鉴定出 11 个化合物, 分别为3β-acetoxyl-20S-dammarane-23-ene-20, 25-diol (1), dammar-24-ene-3β-acetate-20S-ol (2), 3β-acetoxyl-20S, 24R-dammarane-25-ene- 24-hydroperoxy-20-ol (3), β-amyrin acetate (4), betulinic acid (5), morolic acid (6), phillyrin (7), pinoersinol (8), phillygenin (9), 3β-acetyloleanolic acid (10), 3β-hydroxy-labda-8(17), 13E-dien-15-oic acid (11)。我们对化合物 1-3 进行了细胞毒试验, 均显示出较强的抑制胃癌细胞增殖的活性。结论:化合物 1,3, 6, 11 为首次从木樨科植物中分离得到, 其中化合物 2 显示了显著的细胞毒活性。更多还原

【Abstract】 AIM: To investigate the chemical constituents from the fruits of Forsythia suspense (Thunb.) Vahl.. METHODS: Compounds were separated by column chromatography with silica gel, Sephadex LH-20 and ODS. Their structures were elucidated on the basis of physiochemical and spectral analyses. RESULTS: Eleven compounds were obtained from the ethyl acetate fraction, and their structures were elucidated as 3-acety-isofouquierol(1), dammar-24-ene-3β-acetate-20S-ol (2), 3β-acetoxyl-20S, 24R-dammarane-25-ene-24-hydroperoxy-20-ol (3), β-amyrin acetate (4), betulinic acid (5), morolic acid (6), phillyrin (7), pinoersinol (8), phillygenin (9), 3β-acetyloleanolic acid (10), and 3β-hydroxy-labda-8 (17), 13E-dien-15-oic acid (11). We further tested the cytotoxity of the three dammar triterpenoids. They all showed cytotoxity against gastric cancer cells. CONCLUSION: Compounds 1, 3, 6, 11 were isolated from the Forsythia genus for the first time. Compound 2 was the most potent proliferation inhibition compound.更多还原