【摘要】 采用血清学方法、鸡胚培养、病理组织学检查方法 ,从河北省不同年份鸡IBD免疫鸡群中分离和鉴定了HE93,HG96,XN98 和HQ994个毒株。4个毒株回归鸡的死亡率分别为50 % ,40 % ,0 %和10 %。从年份上看 ,IBDV毒力有逐渐减弱的趋势。接种鸡胚并传代 ,前2个毒株均在第一代时鸡胚全部死亡 ,且出现典型的鸡胚病变 ,而后2个毒株 ,初代培养鸡胚不死亡 ,当传至第4代才出现大部分死亡和鸡胚病变。HE93、HG962个毒株可引起易感鸡典型的IBD裸视病变和组织学变化 ,而NX98、HG992个毒株仅引起以法氏囊萎缩为主的变化 ,缺乏法氏囊的充血、水肿、出血等急性病理变化 ,表明IBD病程有非典型化的倾向。用常规的IBDV血清Ⅰ型B -2疫苗对上述4个毒株的保护率分别为10 % ,0 % ,0 %和更多还原

【Abstract】 Serological test,chicken embryo culture and pathotissuelogical examination were performed to isolate and identify four strains of avian IBDV HE93、HG96、XN98 and HQ99 from IBD immunized chicken groups of different ages in Hebei province.There were pathogeracity differences to susceptible chickens among HE93、HG96、XN98 and HQ99 strains,lethality of 50%、40%、0% and 10% respectively.Chicken embryopassage indicated that all death and IBD typical patholoical change at the first generation for HE93 and HG96 strains while for XN98 and HQ99 strain,regular death and IBD pathological changes happened at the fourth generation of consecutive passages.Immane test showed that IBDV serum I commercial vaccine nearly lind no protection to HE93、HG96、XN98 and HQ9 9with the protective rates of 10%、0%、0% and 10% respetively.The HE93、HG96、XN98 and HQ99 are most probably variation strains of IBDV on antigen and pathogeincity.更多还原