【摘要】 采用 RAPD技术 ,利用 2 0 0个引物筛选的 2 2个 10碱基随机引物对桃 182个变种、类型、品种进行 DNA扩增。对扩增位点上的带型分析 ,观察到有的引物在某一位点上明显有带的分离 ,且 S6 5、S4 59、S1 6 7、S6 0 引物在这一位点可以包含一个类群或几个类群全部的供试品种、变种或类型 ,但各类群都没有各自独特的特征带。聚类图分析表明 ,来源无性系的品种遗传一致度最大达 0 .990 ;有的品种也表现出较大遗传一致度的为 0 .985 ;也有的品种的遗传一致度较低 ,范围在 0 .6 86～ 0 .831之间。对桃的分类 ,以水蜜桃、寿星桃、垂枝桃作为类群划分较明晰 ,其它品种的类群划分则较困难 ,从而也说明了原产中国的桃种质资源的遗传多样性丰富更多还原

【Abstract】 By using of RAPD,total genomic DNAs of 182 variants,types and cultivars in Amygdalus persica were amplified with preselected 22 decamer arbitrary primers from 200 primers.Through band analysis of amplified loci,bands of some primers were apparently separated,which of primers S 65 ,S 459 ,S 167 ,S 60 could include all materials of one or some groups,but each group didn’t own unique band.From analysis of UPGMA cluster,two accessions with genetic similarity index 0 990 from one of asexuality were the biggest among the experimented accessions,some accessions which had 0 985 bigger,some ones that had the scale of 0 686~0 831 lower.If the accessions was classified with results of RAPD,of which some ones could divided among honey peach group,Shouxingtao group and weeping peach type.The others were difficult to be classified.So know that genetic diversity of Amygdalus persica was extremely complicated.更多还原