【摘要】 目的：建立甲基丙烯酸-丙烯酸乙酯共聚物（物质的量比1∶1）中残留单体的测定方法。方法：采用高效液相色谱法。色谱柱为Agilent ZORBAX SB-C₁₈（4.6×150 mm, 5μm）,磷酸盐缓冲液（用磷酸溶液调节pH值至2.0）为流动相A,甲醇为流动相B,流速为1.0 mL/min,检测波长为202 nm,柱温为20℃。结果：甲基丙烯酸-丙烯酸乙酯共聚物（物质的量比1∶1）各主峰与相邻杂质峰分离度良好，甲基丙烯酸和丙烯酸乙酯在浓度0.1～2.0μg/mL范围内均与其峰面积呈现良好的线性关系（r²=0.999 9,0.999 4）;精密度和重复性的相对标准偏差（RSD）均小于2%;稳定性试验表明供试品溶液不稳定应临用现配；甲基丙烯酸和丙烯酸乙酯平均加标回收率分别为97.22%（RSD=4.12%）,107.78%（RSD=1.93%）。结论：经方法学验证，此方法具有操作简单、快速灵敏、准确等优点，适用于甲基丙烯酸-丙烯酸乙酯共聚物（物质的量比1∶1）残留单体的测定。更多还原

【Abstract】 Objetive: To establish a high-performance liquid chromatography（HPLC） method for the determination of residual monomers of methacrylic acid-ethyl acrylate copolymer（1∶1）.Methods: The determination was carried out on a Agilent ZORBAX SB-C₁₈（4.6×150 mm, 5 μm） column, with phosphate buffer（by phosphoric acid solution adjust to pH 2.0） as mobile phase A and methanol as mobile phase B.The flow rate was 1.0 mL/min, the UV detection wavelength was set at 202 nm and the column temperature was 20 ℃.Results: The linear range was 0.1～2.0 μg/mL for both methacrylic acid（r²=0.999 9） and ethyl acrylate（r²=0.999 4）;The relative standard deviation of precision and repeatability were less than 2%.The experiments show that the test solution is unstable and should be prepared temporarily.The average recoveries of methacrylic acid and ethyl acrylate were 97.22%,107.78% with the relative standard deviation of 4.12% and 1.93%.Conclusion: The method is validated that it has the advantages of simple operation and accurate results.It can be used for the determination of residual monomers of methacrylic acid-ethyl acrylate copolymer（1∶1）.更多还原