【摘要】 目的:探讨宫颈癌组织中VEGF、Ki67的表达与肿瘤浸润性及淋巴结转移的关系。方法:收集45例手术切除或活检标本,包括正常宫颈组织,宫颈鳞癌组织(按有无淋巴转移、分化及临床分期分组)。进行SP免疫组化染色测定VEGF蛋白、Ki67蛋白的表达。结果:正常宫颈组织VEGF蛋白不表达,宫颈癌组织中VEGF的表达率为48.57%。VEGF阳性表达与分化程度、临床分期、淋巴结转移呈正相关(χ2=10.3001,P<0.01;χ2=10.1521,P<0.01;χ2=5.9063,P<0.05)。Ki67阳性表达与临床分期、分化程度无正相关,与淋巴结转移呈正相关(χ2=2.6662,P>0.05;χ2=0.8649,P>0.05;χ2=11.9442,P<0.01)。结论:VEGF可能在宫颈癌的发生发展和侵袭转移中起重要作用,有望成为判断宫颈癌预后的指标。Ki67可作为反映宫颈癌细胞增殖能力的指标。更多还原

【Abstract】 Objective:To investigate relationship between the expression of VEGF and Ki67 in human cervix squamous cell carcinoma and the tumor invasion or lymph node metastasis.Methods:The expression of VEGF and Ki67 was detected by using S-P immunohistochemical method in 45 cases of cervix tissue including invasive squamous cell carcinoma of the uterine cervix and normaI cervix tissue.Results:No VEGF expression showed in normal cervix tissue, but the VEGF expression rate was 48.57% in cervix carcinoma.The VEGF expression was positively related to the pathological grade, lymph node metastasis and clinical stage(χ2=10.3001,P<0.01;χ2=10.1521,P<0.01;χ2=5.9063,P<0.05).The Ki67 expression showed no relation to the clinical stage and pathological grading,but was positively related to the lymph node metastasis(χ2=2.6662,P>0.05;χ2=0.8649,P>0.05;χ2=11.9442,P<0.01). Conclusion:The expression levels of VEGF and Ki67 might play an important role in carcinogenesis,development,invasion and metastasis of cervical cancer,which could serve as a marker to evaluate the prognosis of squamous cell carcinoma of the uterine cervix,and Ki67 could act as an index in the progress of squamous carcinoma of the cervix.更多还原