【摘要】 目的了解肝素、乙二胺四乙酸二钾(EDTA-K2)抗凝剂及放置时间对于血常规白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白(Hb)、血细胞比容(Hct)、血小板计数(PLT)的影响。方法分别取静脉血经肝素、EDTA-K2抗凝,于即刻,0.5、1、2、3、4、5、6、12h在MET(迈瑞)BC5500血细胞分析仪上以全血模式检测WBC、RBC、Hb、Hct和PLT。结果 WBC在3h内与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05);RBC在6h内与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05),在12h内,以肝素抗凝的静脉血与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05);Hb在12h内与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05);Hct在即刻以肝素抗凝的静脉血与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05),而6h内与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05);PLT在1h内与EDTA-K2抗凝的静脉血即刻结果相比较,差异无统计学意义(P>0.05)。结论血常规测定应使用EDTA-K2抗凝,并在1h内检测完毕。更多还原

【Abstract】 Objective To learn the influence of anticoagulant EDTA-K2 and heparin and standing time on the WBC,RBC,Hb,Hct,PLT result of venous blood.Methods Blood routine tests to anticoagnlated venous blood samples with EDTA-K2 and heparin under room temperature were tested instantly every 0.5,1,2,3,4,5,6,12 h respectively.Results There had no significant difference in WBC within 3 hours compared with the two at instant(P>0.05);There was no significant difference in RBC within 6 hours and 12 hours compared with the two at instant(P>0.05);There was no significant difference in Hb within 12 hours compared with the two at instant(P>0.05);There was significant difference in Hct with the two at instantance(P<0.05).No significant difference in Hct within 6 hours was compared with the two at instant(P>0.05);There was no significant difference in PLT within 1 hour compared with the two at instant(P>0.05).Conclusion Blood routine test should be done within 1 hour using anticoagulant EDTA-K2.更多还原