【摘要】 目的研究类风湿性关节炎(rheumatoid arthritis,RA)患者外周血抗炎性因子及可溶性PD-1(soluble PD-1,SPD-1)分子的检测价值。方法选择2015年1月至2016年12月四川省雅安市人民医院收治的RA患者68例纳入病例组,选择同期健康人68例纳入对照组,以酶联免疫吸附试验检测两组外周血白细胞介素-37(IL-37)、白细胞介素-1(IL-1)、干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF-α)及SPD-1等细胞因子水平,评价病例组患者RA诊断得分,并判断病例组IL-37、SPD-1与RA诊断得分、IL-1、IFN-γ、TNF-α水平的相关性。结果病例组外周血IL-37、SPD-1、IL-1、IFN-γ、TNF-α水平均高于对照组,差异有统计学意义(P<0.05)。病例组外周血IL-37、SPD-1和RA诊断得分呈正相关性(P<0.05);病例组外周血IL-37与IL-1、IFN-γ、TNF-α均呈正相关性(P<0.05);病例组外周血SPD-1与IL-1、IFN-γ、TNF-α均呈正相关性(P<0.05)。结论 RA患者外周血IL-37、SPD-1与RA的严重程度及其他多种炎性因子密切相关,在RA的临床检测中具有较高应用价值。更多还原

【Abstract】 Objective To explore the value of detecting human Interleukin-37(IL-37)and soluble PD-1(SPD-1)in the peripheral blood of patients with rheumatoid arthritis(RA).Methods 68 RA cases treated in Yaan People’s Hospital of Sichuan from January of 2015 to December of 2016 were selected into the case group,while the other 68 healthy cases over the corresponding period were selected into the control group.Enzyme Linked Immunosorbent Assay(ELISA)was used to detect the levels of cytokines including IL-37,Interleukin-1(IL-1),Interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)and SPD-1in the peripheral blood of the two groups.The diagnosis of those RA patients were evaluated and scored.Then the analysis was made to explore whether the levels of IL-37 and SPD-1 were correlated with the RA diagnostic score and the levels of IL-1,IFN-γand TNF-αrespectively.Results The levels of IL-37,SPD-1,IL-1,IFN-γand TNF-αin the case group were significantly higher than those in the control group respectively(P<0.05).The levels of IL-37 and SPD-1 were positively correlated with the RA diagnostic score respectively in the case group(P<0.05).The levels of IL-37 and SPD-1were positively correlated with the levels of IL-1,IFN-γand TNF-αrespectively in the case group(P<0.05).Conclusion The levels of IL-37 and SPD-1in the peripheral blood of patients with RA are correlated with the severity of RA and the other inflammatory factors,so the detection of IL-37 and SPD-1has high application value in the clinical examination of RA.更多还原