【摘要】 [目的]揭示枸杞雄性不育败育的生理生化机制。[方法]通过分析比较枸杞雄性不育株和可育株叶片及花蕾中游离脯氨酸的含量研究枸杞雄性不育性与游离脯氨酸含量的关系。[结果]在减数分裂期和花粉成熟期,雄性不育株YX-1的叶片游离脯氨酸含量高于可育株宁杞1号。叶片游离脯氨酸含量在春梢生长期最高,在展叶期最低。在生殖生长阶段,宁杞1号的叶片游离脯氨酸含量逐渐降低,YX-1的叶片游离脯氨酸含量逐渐升高。随着花蕾的发育,YX-1的花蕾脯氨酸含量逐渐降低,宁杞1号的花蕾脯氨酸含量逐渐升高。在减数分裂时期和花粉成熟期,YX-1的花蕾游离脯氨酸含量明显低于宁杞1号。[结论]不育株叶片游离脯氨酸含量高于可育株,花蕾游离脯氨酸含量低于可育株,可能是导致雄性不育的原因。更多还原

【Abstract】 [Objective] The purpose was to reveal the physiological and biochemical mechanism of male sterility and abortion in Lycium barbarum.[Method] The correlation between male sterility and free proline content in L.barbarum was studied through analyzing and comparing the free proline contents in leaves and flower buds of its male sterile plants and fertile plants.[Result] In meiophase and pollen maturation period,the free proline contents in leaves of male sterile plant YX-1 were higher than that of fertile plant Ningqi 1.Their free proline contents in leaves were highest in the growth period of spring shoots and lowest in leaf-expansion period.In generative growth phase,the free proline contents in leaves of Ningqi 1 decreased gradually and that of YX-1 increased gradually.Along with the development of flower buds,the free proline contents in flower buds of YX-1 decreased gradually and that of Ningqi 1 increased gradually.In meiophase and pollen maturation period,the free proline contents in flower buds of YX-1 were significantly lower than that of Ningqi 1.[Conclusion] The free proline contents of sterile plant leaves were higher than fertile plants,and the free proline contents of flower buds were lower than fertile plants,these may be the reasons of male sterility.更多还原