【作者】 向博文；

【导师】 刘德华；

【作者基本信息】 湖南农业大学 ， 茶学， 2010， 硕士

【摘要】 为了建立显齿蛇葡萄植株再生体系,本研究以湖南农业大学资源资源圃栽植的显齿蛇葡萄为材料,以长约1.0 cm左右的第10至第15节茎段和第1至第5节茎段作茎段外植体(简称P1、P2),以剪除叶尖以下2/3叶片具有小叶叶柄的叶片为叶外植体(简称P3)。研究了影响其植株再生的因素,结果如下：1.显齿蛇葡萄组织培养最佳灭菌方案为：茎叶材料用70%酒精浸泡10S左右后,P1的材料采用0.25%HgCl2水溶液浸泡25min灭菌,P2、P3的材料用0.15%HgCl2水溶液浸泡15 min灭菌。2.P3在添加IBA 0.05 mg/L+BA 2.0 mg/L的MS(含20%蔗糖)培养基培养,愈伤组织诱导率较高；P1、P2在IBA 0.05 mg/L+BAl.5 mg/L的MS(含20%蔗糖)培养基培养,愈伤组织诱导率较高；P1、P2诱导的愈伤组织分化出了畸形胚,但未能直接进一步分化出芽。3.具有腋芽的茎段外植体在培养中,只诱导出了腋芽萌发生长,不产生丛芽；木质化程度高的腋芽萌发率高于木质化程度低的。不具腋芽、具有腋芽基部细胞的茎段外植体,容易诱导出丛生芽,木质化程度低的外植体的丛生芽诱导率显著高于木质化程度高的外植体。4.具小叶叶柄的叶外植体在附加TDZ 0.1 mg/L+IBA 0.5 mg/L的BM(2%蔗糖)培养基培养,诱导出了不定芽。5.具成熟叶的试管苗茎段在MS+IBA 0.05 mg/L培养基上培养较容易诱导生根。6.显齿蛇葡萄的小苗栽至黄泥：细沙=2：1的土壤中,在温度25℃,75%以上湿度下,成活率为93%。更多还原

【Abstract】 In order to building high-performance of Ampelopsis grossedentata (Hand-Mazz) W.T.Wang breeding system, this paper suffered Ampelopsis grossedentata stem and leaf of the Hunan Agricultural University Resources garden, for the material, about 10 to 15 stems and 1 to 5 stems for stem explants (referred to as P1, P2), to cut off the tip and the following 2/3 of leaf petiole of the leaf with the leaflets as explants (referred to as P3). To study the factors affecting of plant regeneration, the results are as follows:1. The Optimal disinfecting programs of Ampelopsis grossedentata tissue culture are as follows:the stem and leaf materials immerse in 75 percent ethanol about 10 seconds, then P1 use 0.25% HgCl2 disinfecting 25 minutes, P2 and P3 use 0.15% HgC12 disinfecting 15 minutes.2. Callus induction of P3 is in BA 2.0mg/L and IBA0.05mg/L with 20% sucrose of MS medium, and callus induction of P1、P2 is in BA1.5 mg/L and IBA 0.05 mg/L with 20% sucrose of MS medium. Callus induction of P1、P2 differentiate the abnormal embryos, but failed to further differentiating the bud.3.Stem segments with axillary bud explants in culture, only induced the growth of axillary buds, do not produce adventitious buds. The germination rate of axillary bud with high lignification degree is superior to low lignification degree.Stem explants with non-axillary bud and axillary bud basal cells are easy to induce multiple shoots.Low lignification explant shoot induction rate was significantly higher than the high degree of lignification explants.4.Leaf explants with leaflets petiole cultured in the BM medium add TDZ 0.1 mg/L, IBA 0.5 mg/L and 2% sucrose, induced the adventitious buds5. The Plantlets stems with mature leaves in the. MS+IBA 0.05 mg/L culture medium induced more easily take root.6.The Ampelopsis grossedentata tube seedlings cuttings in the soil with the mass ratio Wong Nai to sand is 2 to 1, temperature 25℃, humidity over 75%. The survival rate is 93%.更多还原