【作者】 陈大勇；

【导师】 刘淑英；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2009， 硕士

【摘要】 为了探讨不同褪黑素水平对鸡免疫机能的影响,本研究通过控制不同光照时间,将4周龄雏鸡分为3个光照组(6h光照组、12h光照组、18h光照组)控制光照3周后,分三批进行:○1采用流式细胞术检测各组间鸡外周血中CD3+、CD4+、CD8+T淋巴细胞及其亚群和Bu-1a+B淋巴细胞数量的变化;○2采用碳廓清试验利用分光光度计检测单核细胞吞噬反应的变化;○3常规HE染色,显微镜观察鸡主要淋巴器官组织形态学变化,拍照,并用测微尺测量胸腺小体直径、腔上囊小结面积、脾小结直径。结果表明:随着光照时间的延长,由于褪黑素分泌受到抑制,外周血中CD3+、CD4+、CD8+T淋巴细胞及其亚群和Bu-1a+B淋巴细胞数量均有不同程度的降低,且同一批次试验组测定结果与对照组相比较均存在显著差异(p<0.05)或极显著差异(p<0.01)。说明褪黑素对鸡外周血中具有特异性免疫功能的T、B淋巴细胞及其亚群的百分含量有显著的影响,提示:褪黑素能够通过增加机体外周血中T、B淋巴细胞及其亚群的百分含量,提高机体的免疫机能。单核细胞吞噬指数有不同程度的降低,且同一批次试验组单核细胞吞噬指数与对照组相比较均存在显著差异(p<0.05)或极显著差异(p<0.01)。说明褪黑素对鸡外周血中具有非特异性免疫功能的单核细胞的吞噬作用有显著影响。提示:褪黑素能够通过提高机体外周血中单核细胞的吞噬能力,增强机体的免疫机能。同批随着光照时间的延长,胸腺小体直径、腔上囊小结面积、脾小结直径都有不同程度的降低,测量数据与对照组相比较均存在显著差异(p<0.05)或极显著差异(p<0.01)。且镜下观察:6h光照组与对照组、18h光照组相比较,胸腺小体直径增加,数量增多,淋巴细胞致密;腔上囊淋巴小结面积增大,淋巴细胞排列密集;脾小结直径增加,生发中心明显,脾动脉周围淋巴鞘增厚。说明不同光照条件下褪黑素能够显著影响雏鸡免疫器官的组织结构,提示:褪黑素能够影响机体主要淋巴器官的发育,从而影响其对淋巴细胞的分泌,进而影响整个机体免疫机能。更多还原

【Abstract】 In order to study the effect of different levels of melatonin on the immune function of chicken, three groups of 4-weeks chickens under different lighting conditions were divided into 6h short lighting, 18h long lighting and 12h control groups, After three weeks of control lighting,three tests were conducted contents of CD3+,CD4+,CD8+T cells and Bu-1a+B cells were examined in peripheral blood by using flow cytometry in different lighting conditions;change of monocyty macrophage phagocytic reaction were examined by spectrophotometer and carban particles clearance test ,and dyed with HE in the routine method, observed and photographed under the microscope, and finally stage micrometer scale was used to measure thymic corpuscle diameter, bursa of fabricius nodule area and splenic corpuscle diameter. The result indicated: Contents of CD3+,CD4+,CD8+T cells and Bu-1a+ B cells were magnified significantly with the lighting time increasing and due to the level of the melatonin inhibited in the long time lighting. Result were significantly differen(tp<0.05)or quite significantly differen(tp<0.01)in comparison with that in the control group of same batch. These results showed that the levels of melatonin have significantly affected on the quantities of T,B lymphocytes and their subtypes. It was indicated that the melatonin could improve the immune function by increasing the percentage content of T, B lymphocytes and their subtypes in the peripheral blood , Monocytes macrophage function phagocytic index were magnified significantly. Result were significantly different(p<0.05)or quite significantly different(p<0.01)in comparison with that in the control group of same batch. These results showed that the levels of melatonin have significantly affected on the monocytes macrophage function phagocytic index. It was indicated that the melatonin could enhance the immune function by increasing the monocyte phagocytic capacity in the peripheral blood, furthermore, With the lighting time increasing, thymic corpuscle diameter, bursa of fabricius nodule area and splenic corpuscle diameter index were decreased in same batch. Result were significantly different (p<0.05 or P<0.01) compared with the control group. The observation under the microscope showed that the diameter and number of thymic corpuscle in 6h lighting group increased and the lymphocytes become denser ;the bursa of fabricius nodule area enlarge and lymphocytes were in denser arrangement; the diameter of the splenic corpuscle increased, the germinal center of splenic corpuscle become obvious, and the periarterial lymphatic sheaths thickened. The results showed that different levels of melatonin have significant effect on the tissue structure of chickens’lymphoid organs under different lighting conditions, It was indicated that melatonin could affect the development of the main lymphoid organs, then affect the secretion of lymphocytes, thereby influence the entire immune function.更多还原