【摘要】 为了提高共轭亚油酸(CLA)在耶氏解脂酵母重组菌株中的产量,选择YEA、YPD、YP2D4、YNBDO2和YNBDL25种培养基,对其进行摇瓶发酵,分析其生长状态,并利用气相色谱法测定菌体中的总脂含量及脂肪酸组成。结果表明:培养基中充足的碳源和氮源是提高菌体生物量及重组蛋白表达量的前提;在培养基中添加脂肪酸显著提高了菌体中的总脂含量和t10,c12-CLA产量;在5种培养基中,YNBDL2是最适合t10,c12-CLA合成的培养基,其培养基中t10,c12-CLA产量为185.8 mg/L,是YPD培养基中t10,c12-CLA产量的13倍。更多还原

【Abstract】 In order to increase the yield of conjugated linoleic acid(CLA) in recombinant Yarrowia lipolytica,five mediums(YEA,YPD,YP2D4,YNBDO2 and YNBDL2) were used for flask fermentation to analyze its growing state.Total lipid content and fatty acid composition of strains in different mediums were determined by gas chromatography.The results showed that sufficient carbon source and nitrogen source in medium ensured high levels of biomass and recombinant protein expression.Adding fatty acid to medium had significantly positive effects on lipid accumulation and biosynthesis of t10,c12-CLA.Among five mediums,YNBDL2 was the best fermentation medium for t10,c12-CLA production by Yarrowia lipolytica,and the yield of t10,c12-CLA was 185.8 mg/L,which was thirteen times as high as that produced in YPD.更多还原