【摘要】 目的:研究酶解与溶剂联合提取银杏叶中黄酮类化合物的最佳工艺条件。方法:以黄酮得率为考察指标,通过单因素试验及正交实验确定最佳提取工艺。结果:酶解-溶剂联合提取银杏黄酮的最佳工艺条件是:酶解pH值为5.7,酶解浓度为0.4 mg/mL,酶解温度为45℃,酶解时间为2h,液固比为20∶1,提取温度为70℃,提取时间为2h,乙醇浓度为70%,银杏黄酮平均得率为3.62%。结论:采用酶解-溶剂联合提取银杏黄酮,工艺方法简单,可大大提高原料利用率。更多还原

【Abstract】 Objective:To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba.Methods:To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids.Results:The optimum extraction conditions were established as follows:pH5.7,the concentration of enzyme 0.4 mg/mL,enzymatic hydrolysis temperature 45℃,enzymatic hydrolysis time 2h,the rate of liquid to material 20∶1,extraction temperature 70℃,extraction time 2h,the concentration of ethanol 70%,the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%.Conclusion:The method is simple and can increase material utility effectively.更多还原