【摘要】 建立了超高效液相色谱-二极管阵列检测器（UPLC-DAD）波长切换法同时测定替吉奥胶囊中的替加氟（1）、吉美嘧啶（2）和奥替拉西钾（3）3种主要成分。采用Accucore-C₁₈色谱柱,以0.01 mol/L磷酸二氢钾溶液（pH 4.0）∶甲醇（90∶10）为流动相,切换波长检测,检测波长分别为240 nm（0～3 min,3）,214 nm（3～6 min,2）,272 nm（6～10 min,1）。结果 1～3分别在10～100、2.9～29和9.8～98μg/ml范围内线性关系良好,1～3的平均回收率（n=9）分别为97.8%、99.1%和99.5%,RSD分别为2.77%、1.56%和1.72%。更多还原

【Abstract】 A UPLC-DAD wavelength switching method was established for the simultaneous determination of tegafur（1）, gimeracil（2） and potassium oxonate（3） in Tiji’ao capsules. The Accucore-C₁₈ column was used, with the mobile phase of 0.01 mol/L potassium dihydrogen phosphate solution（pH 4.0）∶methanol（90∶10）, at the detection wavelengths of 240 nm（0-3 min, 3）, 214 nm（3-6 min, 2） and 272 nm（6-10 min, 1）. The linear ranges for 1-3 were 10-100, 2.9-29 and 9.8-98 μg/ml, respectively. The average recoveries were 97.8%, 99.1% and 99.5%, with RSDs of 2.77%, 1.56% and 1.72%, respectively. This method is proved to be valid for the quality control of Tiji’ao capsules.更多还原