【摘要】 目的:研究博落回Macleaya cordata的生物碱成分及其抗肿瘤活性。方法:采用乙醇提取和液液萃取等方法提取,硅胶,反相ODS,Sephadex LH-20柱色谱,HPLC等分离技术分离其生物碱成分,利用MS,NMR等光谱技术鉴定化合物结构,采用MTT法测定化合物对MCF-7及SF-268肿瘤细胞株的细胞毒活性。结果:从博落回中分离鉴定了12种生物碱,分别为maclekarpine E(1),6-丙酮基二氢白屈菜红碱(2),岩黄连灵碱(3),6-丙酮基二氢血根碱(4),O-methylzanthoxyline(5),6-甲氧基二氢血根碱(6),spallidamine(7),6-羟基二氢白屈菜红碱(8),arnotianamida(9),二氢血根碱(10),原阿片碱(11),别隐品碱(12)。结论:化合物1,3,7~9为首次从该植物中分离得到,化合物5为新天然产物。药理实验表明,化合物6对MCF-7及SF-268细胞株具有显著的细胞毒活性,IC50值分别为0.61,0.54μmol·L-1。更多还原

【Abstract】 Objective: To study the alkaloids of Macleaya cordata and their anti-tumor activities. Method: Alcohol and liquidliquid extraction were used methods were used to extract the alkaloids constituents,and silica gel,reverse-phase octadecylsilyl( ODS),sephadex LH-20 chromatographic methods and HPLC were applied to isolate and purify compounds. MS,NMR spectroscopic methods were used to determine their structures. Furthermore,the cytotoxicity of these chemical components for MCF-7 and SF-268 cell lines was measured by MTT method. Result: Twelve alkaloids were isolated from the fruits of M. cordata,and their structures were identified as: maclekarpine E( 1),6-acetonyldihyrochelerythrine( 2),cavidilinine( 3),6-acetonyldihyrosanguinnarine( 4),O-methylzanthoxyline( 5),6-methoxy-dihydrosanguinarine( 6),spallidamine( 7),6- hydroxyldihydrochelerythrine( 8),arnotianamida( 9),dihydrosanguinarine( 10),protopine( 11),and cryptopine( 12). Conclusion: Compounds 1,3,7-9were isolated from M. cordata for the first time,and compound 5 is a new natural product. The results of cytotoxic assay indicated that compound 6 showed strong cytotoxicity against MCF-7 and SF-268 cell lines with IC50 values of 0. 61 μmol·L- 1and 0. 54 μmol·L- 1,respectively.更多还原