【摘要】 对蜜环菌Armillaria mellea发酵培养物的化学成分进行研究。采用硅胶、Sephadex LH-20柱色谱等方法进行分离纯化,根据波谱数据及与对照品对照鉴定化合物结构。从蜜环菌发酵培养物中分离鉴定了10个化合物,分别为2-羟基-4-甲氧基-6-甲基苯甲酸(1),苔藓酸(2),蜜环菌戊素(3),麦角甾醇(4),染料木素(5),大豆素(6),胡萝卜苷(7),染料木苷(8),尿嘧啶(9)和甘露醇(10)。化合物1～10均为首次从蜜环菌发酵培养物中分离得到,其中,化合物1～4,6,10在蜜环菌菌丝体或菌索中已有报道。通过二维核磁波谱确证,首次报道化合物3以DMSO为溶剂的氢、碳谱数据,是对该类成分波谱数据的补充。更多还原

【Abstract】 Ten compounds were isolated from cultures of the fungus Armillaria mellea by silica gel and Sephadex LH-20 column chromatographies.Their structures were identified by spectroscopic data analysis and compared their spectroscopic data with those reported in the literature as 2-hydroxy-4-methoxy-6-methylbenzoic acid(1),orsellinic acid(2),melleolide(3),ergosterol(4),genistein(5),daidzein(6),daucosterol(7),genistin(8),uracil(9) and D-mannitol(10).Compounds 1-10 were isolated from cultures of A.mellea for the first time.Among them,1-4,6 and 10 had been obtained from mycelia or rhizomorphs of A.mellea.The structure of compound 3 was determined by detailed analysis of its 1D and 2D NMR data in the solvent of DMSO for the first time.更多还原