【摘要】 应用免疫组化方法在含有肺鳞癌及其癌前病变的标本上观察了变异型CD44（CD44v）和增殖细胞核抗原（PC－NA）在肺鳞癌发生过程中出现表达的时间和频率。结果癌膏正常支气管上皮细胞未见CD44v表达，在鳞状化生的支气管上皮细胞、轻→中→重度不典型增生、原位癌、浸润癌，CD44v表达的阳性率逐渐升高，阳性细胞也逐渐增多。表达CD44v的不典型增生的支气管上皮，其相应的鳞癌CD44v也呈阳性表达。PCNA的表达也呈现上述特点，但出现表达的时间早于CD44v。结果表明，变异型CD44和PCNA可作为判断肿瘤发生可能性的有用的标记物，在肺鳞癌的早期诊断中具有重要意义。更多还原

【Abstract】 Immunohistochemical analyses for CD44v and PCNA expression in specimens of squamous cell carcinomas of lung with adjacent normal epithelium and/or precancerous lesions from 34 patients were made. Mernbrane ex-pression of CD44v. was present in 0 of 12 specimens with normal mucosa, in 2 of 14 with squamous metaplasia, in 3 of 13 with mild dysplasia, in 5 of 17 with moderate dysplasia, in 12 of 21 with severe dysplasia, in 5 of 9 with car-cinoma in situ, and in 21 of 34 with invasive tumor, while PCNA labeling index was 6. 5± 3. 3, 9. 4± 4. 7, 12. 5±8. 7, 43. 3 ± 14. 9, 68. 1± 9. 1, 74. 6 ± 8. 2, 650 4± 23. 6 respectively in the above mentioned lesions. With the progress of bronchial lesions from mild dysplasia to severe dysplasia to carcinoma in situ and invasive carcinoma,the immunostained cells of CD44v and PCNA increased in number and appeared in the upper cell layers of the epithe-lium. These findings indicate that CD44v expression is detectable in precancerous bronchial lesions and suggest that routine assessment of CD44± and PCNA expression be valuable for the early diagnosis of squamous cell lung carcino-ma.更多还原