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生物酶法催化天然乳胶中过敏蛋白质交联的研究

Crosslinking of allergy proteins in natural rubber latex by transglutaminase

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【作者】 冯屏程必影孙金煜田晓慧王彦超

【Author】 FENG Ping1,CHENG Bi-ying1,SUN Jin-yu2,TIAN Xiao-hui2,WANG Yan-chao1(1.College of Bioengineering,East China University of Science &Technology,Shanghai 200237,China;2.College of Materials Science,East China University of Science &Technology,Shanghai 200237,China)

【机构】 华东理工大学生物工程学院华东理工大学材料科学与工程学院

【摘要】 利用谷氨酰胺转氨酶催化天然乳胶(NRL)水溶性蛋白质(WSP)发生交联,减少天然乳胶中水溶性蛋白质的含量。利用粒径分析、结构表征、十二烷基硫酸钠凝胶电泳实验证明了交联反应的发生。采用这一新技术可以降低天然乳胶制品的过敏性,拓展了其在医疗卫生领域、食品加工等领域的应用空间。

【Abstract】 Water-soluble proteins existed in natural rubber latex can easily cause allergic reaction in people.Microbial Transglutaminase(MTGase)can modify latex proteins by means of covalent crosslinking to reduce the content of water-soluble proteins in natural rubber latex.The allergy proteins were considered to be catalysis of crosslinking reaction with MTGase,which were verified by particle-size analysis,characterization of structure and SDS-PAGE.Using this new technology can reduce allergic reaction of natural rubber latex products and expand the application space in the area of health,food processing and other fields.

【基金】 上海市基础研究重点项目(09JC1404300)
  • 【分类号】TQ331.2
  • 【被引频次】3
  • 【下载频次】98
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