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生物酶法催化天然乳胶中过敏蛋白质交联的研究
Crosslinking of allergy proteins in natural rubber latex by transglutaminase
【摘要】 利用谷氨酰胺转氨酶催化天然乳胶(NRL)水溶性蛋白质(WSP)发生交联,减少天然乳胶中水溶性蛋白质的含量。利用粒径分析、结构表征、十二烷基硫酸钠凝胶电泳实验证明了交联反应的发生。采用这一新技术可以降低天然乳胶制品的过敏性,拓展了其在医疗卫生领域、食品加工等领域的应用空间。
【Abstract】 Water-soluble proteins existed in natural rubber latex can easily cause allergic reaction in people.Microbial Transglutaminase(MTGase)can modify latex proteins by means of covalent crosslinking to reduce the content of water-soluble proteins in natural rubber latex.The allergy proteins were considered to be catalysis of crosslinking reaction with MTGase,which were verified by particle-size analysis,characterization of structure and SDS-PAGE.Using this new technology can reduce allergic reaction of natural rubber latex products and expand the application space in the area of health,food processing and other fields.
【Key words】 natural rubber latex; microbial transglutaminase; water-soluble proteins; allergy; crosslinking;
- 【文献出处】 弹性体 ,China Elastomerics , 编辑部邮箱 ,2012年03期
- 【分类号】TQ331.2
- 【被引频次】3
- 【下载频次】98