【摘要】 本文旨在探讨重症急性胰腺炎(SAP)大鼠肠黏膜屏障、肠道菌群的变化和细菌移位的情况。将健康雄性SD大鼠随机分为对照组(n=10)和实验组(n=14),采用5%牛磺胆酸钠胰胆管逆行注射法制作重症急性胰腺炎模型,24h后处死,测定血清淀粉酶及血浆内毒素水平,观察胰腺、小肠病理改变并评分,检测腹腔脏器细菌移位率,实时荧光定量聚合酶链式反应法(RT-PCR)定量分析肠道内菌群数量。结果显示:实验组大鼠肠腔内大肠埃希菌属计数明显升高(9.72±3.58,P<0.01),乳酸杆菌属(0.67±0.34,P<0.01)和双歧杆菌属(4.59±3.42,P<0.05)计数明显减少,血浆内毒素测定阳性率增高(P<0.01),腹腔脏器细菌移位率升高(P<0.01或P<0.05),胰腺及小肠出现明显病理损伤。表明重症急性胰腺炎大鼠肠黏膜屏障受损,出现肠道菌群失调、细菌及内毒素移位,其与多脏器感染的发生、发展或有关联。更多还原

【Abstract】 This paper is to explore changes of intestinal mucosal barrier,intestinal flora,and bacterial translocation in rats with severe acute pancreatitis(SAP).Twenty four male SD rats were randomly divided into the control group(n=10)and the experimental group(n=14).The model of severe acute pancreatitis of rats was induced by the method of injecting adversely 5% sodium taurocholate into the common biliary-pancreatic duct.All of the rats were killed after 24 hours and the level of the serum amylase and the plasma endotoxin was determined after that.The pathological changes of pancreas and small intestine were observed through hematoxylin-eosin staining(HE staining)and the abdominal viscera bacterial translocation rates were tested.With the method of real-time polymerase chain reaction(RT-PCR)the quantity of the intestinal flora was analyzed.In the control group,the level of Escherichia coli,Lactobacillus and Bifidobacterium were 2.08±1.29,11.04±7.55 and 12.21±4.95,respectively.On the contrast,the level of Escherichia coli in the cecum contents was much higher(9.72±3.58,P<0.01),while the Lactobacillus number was decreased significantly(0.67±0.34,P<0.01),and the Bifidobacterium number was also decreased(4.59±3.42,P<0.05)in the experimental group,so the ratio of Bifidobacterium/Escherichia coli was reversed.Besides,in the experimental group,the plasma endotoxin positive rates and the bacterial translocation rates were much higher(P<0.01 or P<0.05)and the pathology scores of pancreas and small intestines were also significantly higher(P<0.01)than those in the control group.These results indicated that in severe acute pancreatitis rats,the intestinal mucosal barrier was severely damaged and the dysbacteriosis occurs in the intestinal canal.And these might relate to the occurrence and development of multiple organ infection.更多还原