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菠萝皮果胶的提取及结构组成研究
Extraction,structural and compositional characterization of pineapple rind pectin
【摘要】 以菠萝皮为原料,采用漂烫灭酶、酸法提取和醇沉工艺提取果胶。对提取果胶的结构和组成进行分析,并与常见的双子叶植物(苹果、柑橘)果胶进行比较。结果表明:菠萝皮经10min漂烫灭酶后,在料液比1∶15、pH2.0、温度80℃条件下提取100min;提取液经2.5倍体积乙醇在pH3.7醇沉,此条件下果胶的得率为4.68%,半乳糖醛酸含量为68.73%。菠萝皮果胶中含有部分甲氧基和少量乙酰基,FCC滴定法测定其酯化度为48.73%,是一种含乙酰基的低酯果胶。果胶多糖中含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖6种单糖,其组成摩尔比为0.74∶0.79∶1.54∶0.92∶0.84∶1.00,与双子叶植物果胶存在很大差异。
【Abstract】 Pineapple rind pectin was prepared by acid extraction after blenching,followed by alcohol precipitation,and the optimal condition was investigated.The structure of pineapple rind pectin was characterized with monosaccharide composition analysis,methylation analysis,FT-IR spectroscopy,and was compared with pectin from dicotyledon.Pineapple rind was blenched for 10 min and pectin was extracted at pH2.0,80℃,for 100 min with solid-liquid ratio 1∶ 15,and precipitated with ethanol in the ratio 1∶ 2.5(v/v)at pH3.7.The yield,content of galacturonic acid and degree of esterification was 4.68%,68.73% and 48.73%,respectively.FT-IR spectroscopy of pineapple rind pectin suggested the presence of acetyl groups.Pineapple rind pectin consisted of six monosaccharides,and the composition was quite different from dicot pectins.
【Key words】 pineapple rind; pectin; extraction; structure; monosaccharide;
- 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2011年11期
- 【分类号】TS255.1
- 【被引频次】13
- 【下载频次】526