【摘要】 目的探讨幽门螺旋杆菌(Hp)感染与胃息肉患者组织中低氧诱导因子-1α(HIF-1α)含量的相关性。方法胃息肉患者134例,胃癌患者73例,对照组为117例慢性胃炎患者。取胃部不同部位行改良吉姆萨染色镜检Hp;免疫组化法检测HIF-1α蛋白表达;RT-PCR和Western blotting法分别定量检测胃黏膜组织中HIF-1αm RNA和蛋白的表达水平。对3组患者Hp感染情况及HIF-1α含量进行统计分析。结果镜检Hp阳性率最高的是慢性胃炎组(80.34%),其次是胃息肉组中腺瘤性息肉(80.00%)。免疫组化结果显示HIF-1α表达阳性最高的是胃癌组和腺瘤性息肉组,分别有67例(91.78%)和4例(80.00%);RT-PCR结果示胃癌黏膜组织中HIF-1αm RNA含量最高,其次是腺瘤性息肉组;Western blotting结果显示胃癌胃黏膜组织中HIF-1α表达水平最高,其次为腺瘤性息肉组,两组相比差异有统计学意义(P<0.01)。结论胃息肉形成可能与Hp感染有关,胃息肉患者中HIF-1α蛋白表达阳性,但Hp感染与HIF-1α蛋白表达之间的关系对胃息肉的影响还有待进一步研究。更多还原

【Abstract】 Objective To investigate the correlation between helicobacter pylori( Hp) infection and HIF-1α expression in patients with gastric polyps. Methods A total of 134 cases of gastric polyps and 73 cases of gastric cancer were randomly selected as the experimental groups,and 117 cases of chronic gastritis served as the control group. The Hp infection in different parts of gastric polyps was detected with improved Giemsa staining; the protein expression of HIF-1αwas examined with immunohistochemistry; HIF-1α expression at both m RNA and protein levels were assessed with RT-PCR and Western blotting. The status of Hp infection and the levels of HIF-1α expression were statistically analyzed. Results Giemsa staining showed that the highest Hp infection rate appeared in chronic gastritis( 80. 34%),followed by gastric polyps( 80. 00%). Immunohistochemical examination displayed that the highest rate of positive HIF-1α expression occurred in gastric cancer and adenomatous polyp groups,accounting for 91. 78%( 67 cases) and80. 00%( 4 cases),respectively. RT-PCR showed that the highest HIF-1α m RNA content was in stomach mucosa tissue of cancer,followed by that in adenomatous polyp. Western blotting showed that expression level of HIF-1α in gastric mucosa tissue of cancer was the highest,followed by that in adenomatous polyp,with statistically significant difference between the two groups( P < 0. 01). Conclusion Gastric polyps may associate with Hp infection and the HIF-1α protein expression of patients with gastric polyp is positive. However,the relationship between Hp infection and HIF-1α expression that influences the pathogenesis of gastric polyps remains to be further studied.更多还原